NR ALOI
AU Taylor,D.M.; McBride,P.A.
TI Autoclaved, formol-fixed scrapie mouse brain is suitable for histopathological examination, but may still be infective
QU Acta Neuropathologica 1987; 74(2): 194-6
PT journal article
AB Autoclaving Creutzfeldt-Jakob disease (CJD)-infected brain after fixation has been proposed previously as a means of making it safe for handling in the laboratory, while preserving its microscopic integrity. However, the practice which was recommended (126 degrees C for 30 min) would be unlikely to achieve complete decontamination. Autoclaving scrapie mouse brain (an analogue for CJD) to the higher, recommended standard of 134-138 degrees C for 18 min was found to lead to some tissue damage but not sufficient to prevent meaningful qualitative and quantitative study of the histopathological lesions.
MH Animal; Brain/*microbiology/pathology; Fixatives; *Formaldehyde; *Heat; Mice; Mice, Inbred C3H; Prions/*pathogenicity; Scrapie/*microbiology/pathology; Sterilization/*methods
AD Patricia A. McBride (tricia.mcbride@bbsrc.ac.uk), Institute for Animal Health, BBSRC and MRC, Neuropathogenesis Unit, Ogston Building, West Mains Road, Edinburgh EH9 3JF, Scotland, UK
SP englisch
PO Westdeutschland