NR ALYR
AU van Keulen,L.J.M.; Schreuder,B.E.C.; Meloen,R.H.; Mooij-Harkes,G.; Vromans,M.E.W.; Langeveld,J.P.M.
TI Immunohistochemical detection of prion protein in lymphoid tissues of sheep with natural scrapie
QU Journal of Clinical Microbiology 1996 May; 34(5): 1228-31
PT journal article
AB The scrapie-associated form of the prion protein (PrPsc) accumulates in the brain and lymphoid tissues of sheep with scrapie. In order to assess whether detecting PrPsc in lymphoid tissue could be used as a diagnostic test for scrapie, we studied the localization and distribution of PrPsc in various lymphoid tissues collected at necropsy from 55 sheep with clinical scrapie. Samples collected from the spleen, palatine tonsil, ileum, and five different lymph nodes were immunohistochemically stained for PrPsc. PrPsc was found to be deposited in a reticular pattern in the center of both primary and secondary lymphoid follicles. In addition, granules of PrPsc were seen in the cytoplasm in macrophages associated with the lymphoid follicles. In 54 (98%) of the 55 scrapie-affected sheep, PrPsc was detected in the spleen, retropharyngeal lymph node, mesenteric lymph node, and the palatine tonsil. However, only in the palatine tonsils was PrPsc present in a consistently high percentage of the lymphoid follicles. PrP was not detected in any of the lymphoid tissues of 12 sheep that had no neurohistopathological signs of a scrapie infection. We conclude that the tonsils are the best-suited lymphoid tissue to be biopsied for the detection of PrPsc in the diagnosis of clinical scrapie in living sheep.
IN Bei 55 2-5 Jahre alten Schafen mit klinischen Scrapie-Symptomen aus 30 Herden wurde Lymphgewebe aus Milz, Gaumenmandeln, Dünndarm und 5 verschiedenen Lymphknoten entnommen. Durch immunhistochemische Anfärbung markierten die Autoren das scrapieassoziierte Prionprotein in den Zentren primärer und sekundärer lymphoider Follikel. Außerdem fanden sie Prione in Makrophagen an lymphoiden Follikeln. In 54 der 55 Schafe wurde das scrapieassoziierte Prionprotein in der Milz, in Rachenlymphknoten, Dünndarmlymphknoten und den Gaumenmandeln nachgewiesen. Das scrapieassoziierte Prionprotein wurde aber nur in Gaumenmandeln in einem hohen Prozentsatz der lymphoiden Follikel gefunden. Bei keinem der 12 gesunden Kontrollschafe wurde scrapieassoziiertes Prionprotein markiert. Diese Testmethode ist zwar nicht uninteressant, für Routineuntersuchungen ist sie aber zu unangenehm und dauert viel zu lange.
ZR 27
MH Animal; Antibodies; Female; Immunohistochemistry; Lymphoid Tissue/*metabolism/*virology; Male; PrPsc Proteins/immunology/*metabolism; Scrapie/diagnosis/*metabolism/*virology; Sheep/*metabolism/*virology; Tissue Distribution; Tonsil/metabolism/virology
AD L.J.M. van Keulen, Department of Pathobiology and Epidemiology, Institute for Animal Science and Health (ID-DLO), P.O. Box 65, 8200 AB Lelystad, The Netherlands, Tel.: 031-320/238238, Fax: 031-320/238050
SP englisch
PO USA
OR Prion-Krankheiten 8