NR AMAA
AU Verghese-Nikolakaki,S.; Michaloudi,H.; Polymenidou,M.; Groschup,M.H.; Papadopoulos,G.C.; Sklaviadis,T.K.
TI Expression of the prion protein in the rat forebrain - an immunohistochemical study
QU Neuroscience Letters 1999 Sep 3; 272(1): 9-12
PT journal article
AB The cellular prion protein (PrPc) is crucial for the development of transmissible spongiform encephalopathies (TSEs), where the pathogenic scrapie isoform (PrPsc) of the same protein, is considered to be the principal or sole infectious agent. Here, we report findings on PrPc expression in the rat forebrain, using immunohistochemical techniques on free floating sections of 60 microm thickness. Along with neurons and astrocytes in the gray matter, PrPc was detected for the first time in glial cells of the white matter and in cells of circumventricular organs. PrPc positive cellular processes were also found to be closely associated with intraparenchymal blood vessels, often in the form of end feet. Interestingly, PrPc expression was observed in areas where PrPsc deposition in late stages of infection has been earlier reported in the rat and other species.
MH Animal; Blood Vessels/metabolism; Immunohistochemistry/methods; Male; Neuroglia/*metabolism; Neurons/metabolism; PrPc Proteins/*metabolism; Prion Diseases/etiology/physiopathology; Prosencephalon/blood supply/*metabolism; Rats; Rats, Wistar; Reverse Transcriptase Polymerase Chain Reaction; Support, Non-U.S. Gov't
AD Laboratory of Pharmacology, School of Pharmacy, Aristotle University of Thessaloniki, Greece.
SP englisch
PO Irland