NR AMAD
AU Vermuyten,K.; Lowenthal,A.; Karcher,D.
TI Detection of neuron specific enolase concentrations in cerebrospinal fluid from patients with neurological disorders by means of a sensitive enzyme immunoassay
QU Clinica Chimica Acta 1990 Feb 28; 187(2): 69-78
PT journal article
AB An enzyme linked immunosorbent assay (ELISA) for the detection of neuron specific enolase (NSE) in cerebrospinal fluid (CSF) was developed. The sensitivity of the ELISA was less than 1 microgram/ml. This sensitivity is comparable with radioimmunoassays which have the disadvantage that radiolabelled products are used. The developed assay was used to measure cerebrospinal fluid neuron specific enolase (CSF-NSE) levels in 1178 patients with neurological disorders to establish its potential usefulness and clinical application. CSF-NSE levels in this group of patients were independent of sex and no correlation with age was found. CSF-NSE was significantly increased in Creutzfeldt-Jakob disease, meningeal hemorrhage, thrombosis, Guillain-Barre syndrome and in schizophrenia.
MH Animal; Antibodies, Monoclonal/diagnostic use; Enzyme-Linked Immunosorbent Assay; Evaluation Studies; Female; Human; Male; Nervous System Diseases/cerebrospinal fluid/*enzymology; Phosphopyruvate Hydratase/*cerebrospinal fluid; Rabbits; Radioimmunoassay; Reference Values; Schizophrenia/cerebrospinal fluid/enzymology; Sex Factors; Support, Non-U.S. Gov't
AD Laboratory of Neurochemistry, Born-Bunge Foundation, U.I.A., Antwerp, Belgium.
SP englisch
PO Niederlande