NR AMBW
AU Vol'pina,O.M.; Zhmak,M.N.; Oboznaya,M.B.; Titova,M.A.; Koroev,D.O.; Volkova,T.D.; Egorov,A.A.; Ivanov,V.T.
TI [Antibodies against synthetic fragments of the prion protein for the diagnosis of bovine spongiform encephalopathy.]
OT Antitela protiv sinteticheskikh fragmentov prionnogo belka dlia diagnostiki gubkoobraznoi entsefalopatii krupnogo rogatogo skota
QU Bioorganicheskaia Khimiia 2001 Sep-Oct; 27(5): 352-8
PT journal article
AB Seven peptides matching fragments of the prion protein and containing from 17 to 31 amino acid residues were synthesized to obtain antibodies for diagnostics of bovine spongiform encephalopathy. Rabbits were immunized with either free peptides or peptide-protein conjugates to result in sera with a high level of antipeptide antibodies. Immunohistochemical assay revealed sera against four free peptides and a protein-peptide conjugate, which effectively bind to the pathogenic isoform of the prion protein in brain tissue preparations from cattle afflicted with bovine spongiform encephalopathy and do not interact with normal brain preparations. The resulting antipeptide sera can be used in developing a diagnostic kit for bovine spongiform encephalopathy.
MH Amino Acid Sequence; Animal; Antibodies/*immunology; Antibody Specificity; Cattle; Encephalopathy, Bovine Spongiform/*diagnosis/immunology; English Abstract; Immunoassay/methods; Molecular Sequence Data; Peptide Fragments/chemistry/genetics/*immunology; Prions/chemistry/genetics/*immunology; Rabbits; Reagent Kits, Diagnostic
AD Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, GSP Moscow, 117997 Russia. volpina@ibch.ru
SP russisch
PO Russland