NR AMCB
AU Volkov,K.V.; Kurishko,K.; Inge-Vechtomov,S.G.; Mironova,L.N.
TI [Polymorphism of the SUP35 gene and its product in the Saccharomyces cerevisiae yeasts]
OT Polimorfizm gena SUP35 i ego produkta u drozhzhei Saccharomyces cerevisiae
QU Genetika 2000 Feb; 36(2): 155-8
PT journal article
AB The product of the SUP35 gene of the saccharomycete yeast, the translation termination eRF3 factor, can be converted in prion, the heritable determinant of protein nature. The nucleotide sequence of this gene from the strain belonging to Peterhof genetic lines of the yeast Saccharomyces cerevisiae was determined. A comparison of the identified sequence with SUP35 sequences in the database of GenBank allowed the detection of polymorphic sites both in the SUP35 gene and its product. The location of polymorphic sites in the evolutionarily nonconserved N-terminal protein region confirmed that this eRF3 fragment lacks functions vital to life activity. Nevertheless, these sites are located in the vicinity of sites, whose role in the prion conversion of eRF3 has been established. Based on this, natural polymorphism of the primary eRF3 structure is assumed to be connected with the existence of different variants (strains) of its prion analog.
MH English Abstract; Fungal Proteins/*genetics; *Genes, Fungal; Peptide Termination Factors/*genetics; *Polymorphism, Single Nucleotide; Prions/*genetics; Saccharomyces cerevisiae/*genetics
AD Department of Genetics and Breeding, St. Petersburg State University, Russia.
SP russisch
PO Russland