NR AMCL
AU Vorberg,I.; Pfaff,E.; Groschup,M.H.
TI The use of monoclonal antibody epitopes for tagging PrP in conversion experiments
QU Archives of Virology. Supplementum 2000(16): 285-90
PT journal article; review; review, tutorial
AB The key event in the pathogenesis of spongiform encephalopathies is a conformational transition of a normal cellular protein, PrPsen, to its pathological isoform, PrPres. The mechanism of PrPres formation is unknown but is likely to involve a direct interaction between PrPsen and PrPres. The molecular basis of PrPres formation has been studied extensively using transgenic mice and scrapie-infected tissue cultures that express heterologous PrP molecules. However, these experiments are dependant on the discrimination of endogenous host PrP and exogenous PrP molecules. Here we give a short review on the PrP-specific epitopes that have been used for tagging exogenous PrP molecules and present a novel PrP-specific epitope that is well suitable for in vivo and in vitro conversion experiments.
ZR 22
MH Animal; Antibodies, Monoclonal/*immunology; Epitopes/immunology; Hamsters; Mice; PrPc Proteins/*chemistry/*immunology/metabolism; PrPsc Proteins/*chemistry/*immunology/metabolism; Protein Conformation; Scrapie/etiology/*metabolism; Support, Non-U.S. Gov't; Tumor Cells, Cultured
AD Federal Research Center for Virus Diseases of Animals, Tuebingen, Germany.
SP englisch
PO Österreich