NR AMCO
AU Vorbrodt,A.W.; Dobrogowska,D.H.; Meeker,H.C.; Carp,R.I.
TI Immunogold study of regional differences in the distribution of glucose transporter (GLUT-1) in mouse brain associated with physiological and accelerated aging and scrapie infection
QU Journal of Neurocytology 1999 Sep; 28(9): 711-9
PT journal article
AB Distribution of glucose transporter (GLUT-1) in brain microvascular endothelia, representing the anatomic site of the blood-brain barrier (BBB), was studied in adult, physiologically aged, senescence-accelerated prone (SAMP8) and in scrapie-infected mice. Sections of tissue samples obtained from four brain regions (cerebral cortex, hippocampus, cerebellum, and olfactory bulb) and embedded in Lowicryl K4M were exposed to anti-GLUT-1 antiserum followed by gold-labeled secondary antibody. Labelling density was recorded over luminal and abluminal plasma membranes of the microvascular endothelial cells. We found that the density of immunosignals for GLUT-1 in the cerebral cortex showed a tendency toward insignificant diminution according to the following gradation-adult > SAMP8 > scrapie > aged mice-whereas in the hippocampus, this gradation was slightly different: adult > aged > scrapie > SAMP8 mice. In the cerebellum, immunolabelling was insignificantly diminished in aged mice, whereas it was significantly decreased in scrapie-infected and SAMP8 mice. The intensity of labelling of the vascular endothelium in the olfactory bulb was significantly lower than that in other brain regions, showing a slight decrease in the following sequence: adult > aged > scrapie > SAMP8 mice. These findings suggest that the process of aging as well as of related neurodegenerative disease affects unequally the distribution of GLUT-1 in the vasculature of different brain regions.
MH Aging/*pathology; Animal; Brain Chemistry/*physiology; Cerebellum/blood supply/chemistry/pathology; Cerebral Arteries/chemistry; Cerebral Cortex/blood supply/chemistry/pathology; Female; Hippocampus/blood supply/chemistry/pathology; Immunohistochemistry; Male; Mice; Mice, Inbred BALB C; Monosaccharide Transport Proteins/*analysis; Olfactory Bulb/blood supply/chemistry/pathology; Scrapie/*physiopathology; Support, Non-U.S. Gov't
AD New York State Office of Mental Retardation and Developmental Disabilities, Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, Staten Island, New York 10314, USA
SP englisch
PO USA