NR AMDO
AU Wadsworth,J.D.F.; Hill,A.F.; Joiner,S.; Jackson,G.S.; Clarke,A.R.; Collinge,J.
TI Strain-specific prion-protein conformation determined by metal ions
QU Nature Cell Biology 1999 May; 1(1): 55-9
KI Nat Cell Biol. 1999 May;1(1):E7. PMID: 10559871
PT journal article
AB In animals infected with a transmissible spongiform encephalopathy, or prion disease, conformational isomers (known as PrPsc proteins) of the wild-type, host-encoded cellular prion protein (PrPc) accumulate. The infectious agents, prions, are composed mainly of these conformational isomers, with distinct prion isolates or strains being associated with different PrPsc conformations and patterns of glycosylation. Here we show that two different human PrPsc types, seen in clinically distinct subtypes of classical Creutzfeldt-Jakob disease, can be interconverted in vitro by altering their metal-ion occupancy. The dependence of PrPsc conformation on the binding of copper and zinc represents a new mechanism for post-translational modification of PrP and for the generation of multiple prion strains, with widespread implications for both the molecular classification and the pathogenesis of prion diseases in humans and animals.
MH Binding Sites; Brain/metabolism; Copper/*metabolism/pharmacology; Creutzfeldt-Jakob Syndrome/classification/*metabolism; Endopeptidase K; Human; PrPc Proteins/*chemistry/metabolism; PrPsc Proteins/*chemistry/metabolism; *Protein Conformation/drug effects; Support, Non-U.S. Gov't; Zinc/*metabolism/pharmacology
AD MRC Prion Unit, Imperial College School of Medicine at St Mary's, London, UK
SP englisch
PO England