NR AMFG
AU Wang,R.F.; Myers,M.J.; Campbell,W.; Cao,W.W.; Paine,D.; Cerniglia,C.E.
TI A rapid method for PCR detection of bovine materials in animal feedstuffs
QU Molecular and Cellular Probes 2000 Feb; 14(1): 1-5
PT journal article
AB Rapid identification of bovine materials in animal feedstuffs is essential for effective control of a potential source of bovine spongiform encephalopathy. We have developed a rapid method for the detection of the presence of bovine materials in animal feeds. Animal feed samples were prepared by a Chelex-100 treatment method, then subjected to polymerase chain reaction (PCR) detection. The assay can be completed in 2 h including 30 min for sample preparation, 35-65 min for PCR cycling and 30 min for gel electrophoresis. This method is not only rapid, simple and consistent, but also avoids a hazardous waste disposal issue associated with a previously described guanidine thiocyanate (GuSCN) extraction-PCR method.
MH Animal; Animal Feed/analysis/*standards; Cattle/genetics; Comparative Study; DNA Primers; Encephalopathy, Bovine Spongiform/prevention & control/transmission; Meat/analysis; Minerals/analysis; Polymerase Chain Reaction/instrumentation/*methods; Sensitivity and Specificity; Time Factors
AD Microbiology Division, National Center for Toxicological Research, Jefferson, AR 72079, USA. rwang@nctr.fda.gov
SP englisch
PO England