NR AMHU
AU Weiss,S.; Rieger,R.; Edenhofer,F.; Fisch,E.; Winnacker,E.L.
TI Recombinant prion protein rPrP27-30 from Syrian golden hamster reveals proteinase K sensitivity
QU Biochemical and Biophysical Research Communications 1996 Feb 6; 219(1): 173-9
PT journal article
AB PrP27-30 represents the protease-resistant core of the prion protein and was found to be the main component in Scrapie prion preparations. Recombinant (r) PrP27-30 corresponding to aa 90-231 from the Syrian golden hamster prion protein was expressed as a fusion with GST in E. coli and secreted from insect cells infected with recombinant baculoviruses, GST::rPrP27-30 isolated from either system was purified to homogenity by glutathione-Sepharose chromatography. rPrP27-30 from both systems was generated by direct cleavage of GST::rPrP27-30 in the presence of thrombin revealing a molecular weight of 17 kDa. GST::rPrP27-30 as well as the authentic protein rPrP27-30 were identified by immunoblotting employing a polyclonal antibody directed against a peptide corresponding to aa 95-110 of the Syrian golden hamster prion protein. In contrast to scrapie prior PrP27-30, the recombinant proteins GST::rPrP27-30 and rPrP27-30 were both sensitive towards proteinase K, suggesting that the molecules lack infectivity.
ZR 36
MH Animal; Cell Line; Cloning, Molecular; Endopeptidase K; Escherichia coli; Glutathione Transferase/biosynthesis; Hamsters; Mesocricetus; PrP 27-30 Protein/chemistry/*metabolism; Prions/chemistry/isolation & purification/*metabolism; Recombinant Fusion Proteins/biosynthesis/isolation & purification; Recombinant Proteins/chemistry/isolation & purification/metabolism; Serine Endopeptidases/*metabolism; Spodoptera; Substrate Specificity; Support, Non-U.S. Gov't; Thrombin/metabolism; Transfection
AD Laboratorium für Molekulare Biologie-Genzentrum-Institut für Biochemie der LMU München, Germany.
SP englisch
PO USA
OR Prion-Krankheiten 8