NR AMIB
AU Weissmann,C.; Raeber,A.J.; Montrasio,F.; Hegyi,I.; Frigg,R.; Klein,M.A.; Aguzzi,A.
TI Prions and the lymphoreticular system
QU Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences 2001 Feb 28; 356(1406): 177-84
IA http://www.journals.royalsoc.ac.uk/(pkuksk45ynsqzt45ifpjlf45)/app/home/contribution.asp?referrer=parent&backto=issue,7,11;journal,71,225;linkingpublicationresults,1:102022,1
PT journal article; review; review, tutorial
AB Following intracerebral or peripheral inoculation of mice with scrapie prions, infectivity accumulates first in the spleen and only later in the brain. In the spleen of scrapie-infected mice, prions were found in association with T and B lymphocytes and to a somewhat lesser degree with the stroma, which contains the follicular dendritic cells (FDCs) but not with non-B, non-T cells; strikingly, no infectivity was found in lymphocytes from blood of the same mice. Transgenic PrP knockout mice expressing PrP restricted to either B or T lymphocytes show no prion replication in the lymphoreticular system. Therefore, splenic lymphocytes either acquire prions from another source or replicate them in dependency on other PrP-expressing cells. The essential role of FDCs in prion replication in spleen was shown by treating mice with soluble lymphotoxin-beta receptor, which led to disappearance of mature FDCs from the spleen and concomitantly abolished splenic prion accumulation and retarded neuroinvasion following intraperitoneal scrapie inoculation.
ZR 53
MH Animal; Dendritic Cells; Human; Lymphatic System/*physiology; Mice; Mice, Knockout; Peptide Fragments/genetics; Prions/genetics/*metabolism; Reticuloendothelial System/*physiology; Spleen/metabolism
AD Medical Research Council Prion Unit, Imperial College School of Medicine at St Mary's, Norfolk Place, London W2 1PG, UK. c.weissmann@ic.ac.uk
SP englisch
PO England