NR AMKL
AU Whatley,S.A.; Powell,J.F.; Politopoulou,G.; Campbell,I.C.; Brammer,M.J.; Percy,N.S.
TI Regulation of intracellular free calcium levels by the cellular prion protein
QU Neuroreport 1995 Nov 27; 6(17): 2333-7
PT journal article
AB A rat brain synaptosomal model was used to investigate the possible role of the cellular prion protein (PrP) in the regulation of intracellular free calcium levels ([Ca2+i). Treatment of synaptosomes with bacterially derived recombinant human PrP in the range 20-100 micrograms ml-1 resulted in dose-dependent elevations of [Ca2+]i. These increases were dependent on extracellular calcium and were inhibited by gadolinium chloride, a potent blocker of voltage-sensitive calcium channels. Conversely, when calcium channels were activated by synaptosomal depolarization, treatment with monoclonal antibody to PrP in the range 200-320 ng IgG ml-1 resulted in a dose-dependent reduction of [Ca2+i, which was blocked by competition with PrP preparations. These results indicate that PrP is associated with regulation of intracellular free calcium levels through an interaction with voltage-sensitive calcium channels.
IN Das normale zelluläre Prionprotein scheint über spannungsempfindliche Calciumkanäle an der Regulation der intrazellulären Konzentration freien Calciums beteiligt zu sein.
ZR 20
MH Animal; Blotting, Western; Calcium/*metabolism; Calcium Channel Blockers/pharmacology; Calcium Channels/drug effects/metabolism; Cerebral Cortex/drug effects/metabolism; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; Gadolinium/pharmacology; Human; Polymerase Chain Reaction; PrPc Proteins/chemistry/genetics/*pharmacology; Rats; Rats, Wistar; Recombinant Proteins/chemistry/genetics/pharmacology; Support, Non-U.S. Gov't; Synaptic Membranes/drug effects/metabolism; Synaptosomes/drug effects/*metabolism
AD Department of Neuroscience, Institute of Psychiatry, London, UK
SP englisch
PO England
OR Prion-Krankheiten 8