NR AMTQ
AU Xi,Y.G.; Ingrosso,L.; Ladogana,A.; Masullo,C.; Pocchiari,M.
TI Amphotericin B treatment dissociates in vivo replication of the scrapie agent from PrP accumulation
QU Nature 1992 Apr 16; 356(6370): 598-601
KI Nature. 1992 Apr 16;356(6370):560. PMID: 1348569
PT journal article
AB Scrapie and related animal and human disorders are neurodegenerative diseases characterized by the formation of a modified, partly proteinase-resistant protein (PrP) of the host, which tends to aggregate as amyloid fibrils and accumulate in the brain of infected individuals. There is a general consensus that the pathological form of PrP (PrPsc) is essential for the clinical appearance of the disease, but whether it is part of the scrapie agent or a by-product of viral infection is still controversial. Here we report that treatment of scrapie-infected hamsters with amphotericin B delays the accumulation in the brain of the proteinase-resistant portion of PrPsc by about 30 days without affecting scrapie replication. The consequence is that hamsters treated with amphotericin B developed clinical signs of disease later than infected controls. We argue that the proteinase-resistant portion of PrPsc is necessary for the development of the disease but that it is unlikely to be essential for scrapie replication.
MH Amphotericin B/*pharmacology; Animal; Blotting, Western; Brain/drug effects/*microbiology; Hamsters; Mice; PrP 27-30 Protein; PrPsc Proteins; Prions/*drug effects/genetics/isolation & purification/*metabolism; Scrapie/*prevention & control; Support, Non-U.S. Gov't; Virus Replication/*drug effects
AD Istituto di Patologia Generale, Universita Cattolica S, Cuore, Rome, Italy.
SP englisch
PO England
OR Prion-Krankheiten 9