NR AMWG
AU Yokoyama,T.; Itohara,S.; Yuasa,N.
TI Detection of species specific epitopes of mouse and hamster prion proteins (PrPs) by anti-peptide antibodies
QU Archives of Virology 1996; 141(3-4): 763-9
PT journal article
AB Antisera to four synthetic peptides containing the substitutions between mouse and hamster prion proteins (PrPs) were produced in rabbits. The synthetic peptides used represent two mouse (Mo-I: residues 100-115 and Mo-V: residues 199-208) and two hamster PrP subregion sequences (Ha-I: 101-116 and Ha-V: 200-209). All antisera reacted strongly with homologous peptides but either not at all or poorly with heterologous peptides in enzymelinked immunosorbent assay (ELISA). Antisera to Mo-I and Mo-V recognized mouse PrPsc but not hamster PrPsc in western blot analysis (WB) and ELISA. Antisera to Ha-I contain antibodies specific to hamster PrPsc. The results indicate that these regions of PrPsc constitute species-specific epitopes. In contrast to these antisera, the antiserum to Ha-V recognized neither hamster nor mouse PrPsc. In this study, we identified mouse subregion-V as a species-specific epitope.
IN Die Autoren stellten polyklonale Kaninchenantikörper gegen synthetische Peptide her. Diese Peptide entsprachen den Aminosäuren 100-115 bzw. 199-208 des Mausprionproteins und den Aminosäuren 101-116 bzw. 200-209 des Mausprionproteins. Im Western Blot erkannten die Mausantisera nur scrapieassoziiertes Mausprionprotein, die Antikörper gegen das Hamsterpeptid 101-116 erkannten spezifisch das scrapieassoziierte Hamsterprionprotein. Das Serum gegen das Hamsterpeptid 200-209 funktionierte im Western Blot mit scrapieassoziierten Prionproteinen nicht.
ZR 24
MH Amino Acid Sequence; Animal; Antibodies/immunology; Antibody Specificity; Epitopes/*immunology; Hamsters; Mice; Molecular Sequence Data; Peptides/chemical synthesis/immunology; Prions/chemical synthesis/*immunology; Rabbits; Species Specificity; Support, Non-U.S. Gov't
AD National Institute of Animal Health, Ibaraki, Japan.
SP englisch
PO Österreich