NR AMXC
AU Yun,M.; Wu,W.; Hood,L.; Harrington,M.
TI Human cerebrospinal fluid protein database: edition 1992.
QU Electrophoresis 1992 Dec; 13(12): 1002-13
PT journal article
AB Two-dimensional electrophoresis maps of human cerebrospinal fluid proteins are presented in the form of labeled images. 931 protein spots are identified in spinal fluid from a normal volunteer. Distinct spots that represent variants of the same protein, especially posttranslational modifications, are estimated to reduce the 931 different spots to < 200 different proteins. 248 spots of 29 protein groups have been identified and are indicated on enlargements of specific gel regions. The distribution of protein abundance, mass, charge and shape characteristics of these normal 931 spinal fluid spots are graphically profiled. Analysis of the shape parameter "vertical height: width ratio" reveals that a ratio > 3.5 correlates with glycoproteins, enabling their identification simply by image analysis. Proteins that are not present on the normal map, but appear in spinal fluid in patients with schizophrenia and Creutzfeldt-Jakob disease are illustrated on additional maps.
IN Bei von der Creutzfeldt-Jakob-Krankheit betroffenen Menschen tauchen in der Rückenmarksflüssigkeit Proteine auf, die dort normalerweise nicht zu finden sind.
MH Cerebrospinal Fluid Proteins/*analysis; Creutzfeldt-Jakob Syndrome/metabolism; *Databases, Factual; *Electrophoresis, Gel, Two-Dimensional; Human; Peptide Mapping/methods; Reference Values; Schizophrenia/metabolism
AD Division of Biology 139/74, California Institute of Technology, Pasadena 91125.
SP englisch
PO Deutschland