NR ANBC
AU Zulianello,L.; Kaneko,K.; Scott,M.R.D.; Erpel,S.; Han,D.; Cohen,F.E.; Prusiner,S.B.
TI Dominant-negative inhibition of prion formation diminished by deletion mutagenesis of the prion protein
QU Journal of Virology 2000 May; 74(9): 4351-60
PT journal article
AB Polymorphic basic residues near the C terminus of the prion protein (PrP) in humans and sheep appear to protect against prion disease. In heterozygotes, inhibition of prion formation appears to be dominant negative and has been simulated in cultured cells persistently infected with scrapie prions. The results of nuclear magnetic resonance and mutagenesis studies indicate that specific substitutions at the C-terminal residues 167, 171, 214, and 218 of PrPc act as dominant-negative, inhibitors of PrPsc formation (K. Kaneko et al., Proc. Natl. Acad. Sci. USA 94:10069-10074, 1997). Trafficking of substituted PrPc to caveaola-like domains or rafts by the glycolipid anchor was required for the dominant-negative phenotype; interestingly, amino acid replacements at multiple sites were less effective than single-residue substitutions. To elucidate which domains of PrPc are responsible for dominant-negative inhibition of PrPsc formation, we analyzed whether N-terminally truncated PrP(Q218K) molecules exhibited dominant-negative effects in the conversion of full-length PrPc to PrPsc. We found that the C-terminal domain of PrP is not sufficient to impede the conversion of the full-length PrPc molecule and that N-terminally truncated molecules (with residues 23 to 88 and 23 to 120 deleted) have reduced dominant-negative activity. Whether the N-terminal region of PrP acts by stabilizing the C-terminal domain of the molecule or by modulating the binding of PrPc to an auxiliary molecule that participates in PrPsc formation remains to be established.
MH Animal; Cell Membrane/metabolism; Mice; Mutagenesis; Phenotype; PrPc Proteins/*biosynthesis/genetics; PrPsc Proteins/*biosynthesis/genetics; Prions/genetics/*metabolism; Protein Processing, Post-Translational; Support, Non-U.S. Gov't; Support, U.S. Gov't, P.H.S.; Tumor Cells, Cultured
AD Institute for Neurodegenerative Diseases, University of California, San Francisco, California 94143, USA
SP englisch
PO USA