NR ANDV
AU Baker,C.A.; Manuelidis,L.
TI Unique inflammatory RNA profiles of microglia in Creutzfeldt-Jakob disease
QU Proceedings of the National Academy of Sciences of the United States of America 2003 Jan 21; 100(2): 675-9
IA http://www.pnas.org/cgi/content/full/100/2/675
PT journal article
AB Previous studies in Creutzfeldt-Jakob disease (CJD) have shown that myeloid cells in the periphery as well as derivative microglial cells in the brain are infectious. Microglia can show an activated phenotype before prion protein (PrP) pathology is detectable in brain, and isolated infectious microglia contain very little PrP. To find whether a set of inflammatory genes are significantly induced or suppressed with infection, we analyzed RNA from isolated microglia with relevant cDNA arrays, and identified approximately 30 transcripts not previously examined in any transmissible spongiform encephalopathy. This CJD expression profile contrasted with that of uninfected microglia exposed to prototypic inflammatory stimuli such as lipopolysaccharide and IFN-gamma, as well as PrP amyloid. These findings underscore inflammatory pathways evoked by the infectious agent in brain. Transcript profiles unique for CJD microglia and other myeloid cells provide opportunities for more sensitive preclinical diagnoses of infectious and noninfectious neurodegenerative diseases.
MH Animals; Cells, Cultured; Creutzfeldt-Jakob Syndrome/*metabolism; *Gene Expression Profiling; Inflammation/*metabolism; Interferon Type II/pharmacology; Lipopolysaccharides/pharmacology; Mice; Microglia/*metabolism; Oligonucleotide Array Sequence Analysis; PrPsc Proteins/pharmacology; Research Support, U.S. Gov't, P.H.S.; Reverse Transcriptase Polymerase Chain Reaction
AD Section of Neuropathology, Yale University School of Medicine, 333 Cedar Street, FMB 11, New Haven, CT 06510, USA
SP englisch
PO USA
OR Prion-Krankheiten 1