NR ANER
AU Head,M.W.; Northcott,V.; Rennison,K.; Ritchie,D.; McCardle,L.; Bunn,T.J.R.; McLennan,N.F.; Ironside,J.W.; Tullo,A.B.; Bonshek,R.E.
TI Prion protein accumulation in eyes of patients with sporadic and variant Creutzfeldt-Jakob disease
QU Investigative Ophthalmology and Visual Science 2003 Jan; 44(1): 342-6
PT journal article
AB PURPOSE: Creutzfeldt-Jakob disease (CJD) primarily affects the brain. This study was conducted to assess the possible involvement of the eye in sporadic and variant CJD by testing for the presence of the disease-associated, protease-resistant isoform of the prion protein (PrPsc) in ocular tissue. METHODS: Human eyes from donors with CJD and non-prion neurodegenerative disease control eyes were studied. In situ hybridization and Western blot analysis were used to determine the normal pattern of cellular prion protein (PrPc) expression. Western blot analysis and immunohistochemistry were then used to determine the localization, abundance, and isotype of PrPsc in eyes in CJD. RESULTS: PrPc was expressed in the nuclear layers of the retina. In both the sporadic and variant forms of CJD, PrPsc accumulated throughout the synaptic layers of the retina. The levels of PrPsc found in the retina were comparable with those found in the brain. Lower levels of PrPsc could be found in the optic nerve, but no PrPsc was detectable in other ocular tissues. The glycoform ratio of PrPsc in the retina did not correspond to that found in the brain. CONCLUSIONS: Presumptive centrifugal spread of PrPsc from the brain through the optic nerve occurs in two major types of CJD. PrPsc is a marker of CJD infectivity. Given that routine decontamination may not remove PrPsc from surgical instruments, a careful risk assessment should be made of possible iatrogenic spread of sporadic and variant CJD after surgery to the retina or optic nerve.
IN Das normale zelluläre Prionprotein wird von den Lichtsinneszellen (Outer Nuclear Layer) sowie von den horizontalen und bipolaren Verschaltungszellen (Inner Nuclear Layer) der Retina exprimiert. Nicht nur bei der neuen Variante, sondern auch bei sogenannter sporadischer Creutzfeldt-Jakob-Krankheit fand man das krankheitstypische PrPsc in den synaptischen Lagen der Retina oberhalb und unterhalb der inneren Zellkernschicht in Konzentrationen, die mit denen im Gehirn vergleichbar waren. Geringere Mengen PrPsc wurden auch im Sehnerven gefunden, kleinere PrPsc-Fragmente gab es in den Augenlinsen, aber man fand kein PrPsc in den anderen Schichten der Augen. Das Verhältnis der unterschiedlich stark glykosylierten Formen des Prionproteins PrPsc war in den Retinas anders als in den untersuchten Gehirnen. Statt einer starken Dominanz der zweifach glykosylierten Form fand man in der Retina eine gleichmäßigere Verteilung mit nur etwas weniger einfach glykosyliertem PrPsc.
MH Blotting, Western; Brain/metabolism; Creutzfeldt-Jakob Syndrome/*metabolism/pathology/transmission; Human; Immunoenzyme Techniques; In Situ Hybridization; Optic Nerve/metabolism; PrPsc Proteins/*metabolism; Retina/*metabolism/pathology; Retinal Diseases/*metabolism; Support, Non-U.S. Gov't
AD Mark W. Head, Victoria Northcott, Kathleen Rennison, Diane Ritchie, Linda McCardle, Tristan J. R. Bunn, Neil F. McLennan, James W. Ironside, National Creutzfeldt-Jakob Disease Surveillance Unit and Department of Pathology of the University of Edinburgh, Scotland, United Kingdom; Andrew B. Tullo, Richard E. Bonshek, Academic Department of Ophthalmology, Manchester Royal Eye Hospital, Manchester, United Kingdom
SP englisch
PO USA