NR ANKE
AU Schwaiger,K.
TI About the occurrence of Transmissible Spongiform Encephalopathies and bacterial CNS infections in roe deer, red deer and chamois in Bavaria
OT Zum Vorkommen Transmissibler Spongiformer Enzephalopathien und bakterieller ZNS-Infektionen beim Reh-, Rot- und Gamswild in Bayern
QU Digitale Inaugural-Dissertation zur Erlangung der tiermedizinischen Doktorwürde der Tierärztlichen Fakultät der Ludwig-Maximilians-Universität München von Karin Schwaiger aus Moosburg, München, 18. Juli 2003
IA http://edoc.ub.uni-muenchen.de/archive/00001166/01/Schwaiger_Karin.pdf
PT Dissertation
AB
Brain samples of 849 wild ruminants (654 roe deer, 189 red deer and 6 chamois) from Bavaria were examined for the occurrence of TSE and encephalopathies caused by bacteria, respectively. For this, the following investigations were carried out:
- General bacteriological investigation (aerobic incubation on blood, standard I nutrient and Gassner Agar)
- Detection of Listeria spp. by selective procedures
-- Cultural isolation and biochemical identification with the BBL crystal ID system
-- Detection of the iap gene using real time PCR (Lightcycler)
- Detection of PrPres by ELISA technique (BioRad)
- Histological investigation
-- HE staining
-- Immunohistochemistry (mAb L42)
Using conventional bacteriological methods, 464 different bacteria were isolated. 229 of them could be differentiated at the genus level and 235 at the species level. Totally, 35 different bacteria species were isolated, most frequently Micrococcus spp., Bacillus spp. and E. coli. Listeria spp. were detected in 55 brain samples (49 from roe deer, 5 from red deer and 1 from chamois). 52 isolates were identified with the BBL crystal ID system as Listeria monocytogenes and 3 as Listeria grayi ssp. murrayi. As the iap gene was detected in all strains, the correctness of the BBL result concerning L. grayi ssp. murrayi must be scrutinised. Analysis of the geographical distribution of the Listeria findings indicate a regional aggregation in Unterfranken (prevalence for roe deer: 17,8 %, versus 6,6 % in Oberbayern-Schwaben, 6,1 in Niederbayern-Oberpfalz and 0 % in Oberfranken-Mittelfranken).
The histological investigation (HE staining) of 87 tissue samples contaminated with encephalitis relevant bacteria showed inflammation of different severity (e. g. perivascular infiltration (n = 26) or (meningo)encephalitis (n = 13)) in 41 cases.
The validation of the ELISA technique for detecting prionprotein from wild ruminants pointed out that the examination of brain of wild ruminants lead to lower extinctions than that of bovine brain samples. Due to the fact that brain homogenate of 2 known CWD positive wapitis showed definite positive results, all samples were analysed with this test: There were no indications for the occurrence of TSE in any sample. In addition, immunohistochemical investigations of 10 % of all samples did not show PrPres.
Due to the large number of samples, the prevalence of TSE can be quoted under 0,5 % for roe deer and under 1,5 % for red deer with a certainty of 95 %.
AD Karin Schwaiger, Wissenschaftszentrum für Ernährung, Landnutzung und Umwelt der Technischen Universität München, Lehrstuhl für Tierhygiene
SP deutsch
PO Deutschland