NR ANUQ
AU Kaneider,N.C.; Kaser,A.; Dunzendorfer,S.; Tilg,H.; Wiedermann,C.J.
TI Sphingosine kinase-dependent migration of immature dendritic cells in response to neurotoxic prion protein fragment
QU Journal of Virology 2003 May; 77(9): 5535-9
PT journal article
AB The concept that circulating dendritic cells mediate neuroinvasion in transmissible spongiform encephalopathies received strong support from recent observations that prion protein is expressed in myeloid dendritic cells. We observed that prion protein fragment 106-126 is a chemoattractant for monocyte-derived immature but not mature dendritic cells. Signaling events in chemotaxis involved enzymes downstream of G(q) protein and were inhibited by blockade of sphingosine kinase, suggesting transactivation of sphingosine-1-phosphate-dependent cell motility by prion protein.
MH Animal; Cells, Cultured; *Chemotaxis/drug effects; Culture Media; Dendritic Cells/cytology/immunology/*physiology; Human; Mice; Peptides/chemical synthesis/chemistry/pharmacology; Phosphotransferases (Alcohol Group Acceptor)/*metabolism; Prions/chemistry/*pharmacology; *Signal Transduction
AD Division of General Internal Medicine. Division of Gastroenterology, Department of Internal Medicine, University of Innsbruck, A-6020 Innsbruck, Austria.
SP englisch
PO USA