NR AOBW
AU Ratzka,P.; Döhlinger,S.; Cepek,L.; Steinacker,P.; Arlt,S.; Jacobi,C.; Schröter,A.; Wiltfang,J.; Prange,H.; Kretzschmar,H.A.; Poser,S.; Otto,M.
TI Different binding pattern of antibodies to prion protein on lymphocytes from patients with sporadic Creutzfeldt-Jakob disease
QU Neuroscience Letters 2003 May 29; 343(1): 29-32
PT journal article
AB In Creutzfeldt-Jakob disease (CJD), progressive neuronal cell death probably occurs as a result of a change in conformation of the physiological prion protein (PrPc). There is evidence of participation of the lymphatic system and in particular of lymphocytes in the intracorporeal transportation of the pathological prion protein (PrPsc) in new variant CJD and scrapie. Using fluorescence cytometry, we investigated a possible alteration of PrPc on lymphocytes of patients with sporadic CJD. We demonstrated a significantly lower binding pattern of antibodies (3F4) against physiological prion protein to lymphocytes of patients with sporadic CJD (n=16) compared with control patients. In contrast this difference was not found on platelets (n=23). For the first time we were able to present a measurable difference of antibody binding on lymphocytes of patients with CJD. One interpretation of this finding is that lymphocytes patrolling the brain bind and transport PrPsc which has a lower binding affinity for the antibodies directed against physiological PrP.
MH Adult; Aged; Aged, 80 and over; Antibodies, Monoclonal; Blood Platelets/immunology/*metabolism; Case-Control Studies; Comparative Study; Creutzfeldt-Jakob Syndrome/immunology/*metabolism; Female; Flow Cytometry/methods; Human; Lymphocytes/immunology/*metabolism; Male; Middle Age; Nervous System Diseases; PrPc Proteins/immunology/*metabolism; Prions/immunology/metabolism; Protein Binding; Reference Values; Support, Non-U.S. Gov't
AD Department of Neurology, University Hospital Göttingen, Robert-Koch Strasse 40, D-37070 Göttingen, Germany. peter.ratzka@t-online.de
SP englisch
PO Irland