NR AOCG
AU Gould,D.H.; Voss,J.L.; Miller,M.W.; Bachand,A.M.; Cummings,B.A.; Frank,A.A.
TI Survey of cattle in northeast Colorado for evidence of chronic wasting disease: geographical and high-risk targeted sample.
QU Journal of Veterinary Diagnostic Investigation 2003 May; 15(3): 274-7
PT journal article
AB A geographically targeted survey of potentially high-risk, adult cattle in chronic wasting disease (CWD)-endemic areas in Colorado was initiated to assess the possibility of the spread of CWD from deer to cattle under natural conditions. Surveyed cattle were sympatric with free-roaming deer in geographically defined areas where CWD occurs and where CWD prevalence has been estimated. To qualify for inclusion in the survey, cattle had to be at least 4 years old and had to have spent a minimum of 4 years in surveyed areas. Brains from culled cattle were examined microscopically and immunohistochemically for tissue alterations indicative of a transmissible spongiform encephalopathy (TSE). Two hundred sixty-two brains were suitable for evaluation and were found to lack changes indicative of a TSE infection. Prion deposition was not demonstrable using a method involving formic acid and proteinase-K treatment before application of monoclonal antibody to bovine prion protein (F99/97.6.1). Some incidental neuropathologic changes unrelated to those of TSEs were detected. Findings from this study suggest that large-scale spread of CWD from deer to cattle under natural range conditions in CWD-endemic areas of northeast Colorado is unlikely.
MH Animal; Brain/metabolism/pathology; Cattle; Cattle Diseases/*diagnosis/*epidemiology/transmission; Colorado/epidemiology; Data Collection; Geography; Risk; Support, Non-U.S. Gov't; Support, U.S. Gov't, Non-P.H.S.; Wasting Disease, Chronic/*diagnosis/*epidemiology/transmission
AD Department of Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Ft. Collins, CO 80523, USA
SP englisch
PO USA