NR AOLF
AU Kettlun,A.M.; Collados,L.; Garcia,L.; Cartier,L.R.; Wolf,M.E.; Mosnaim,A.D.; Valenzuela,M.A.
TI Matrix metalloproteinase profile in patients with Creuztfeldt-Jakob disease
QU International Journal of Clinical Practice 2003 Jul-Aug; 57(6): 475-8
PT journal article
AB Preliminary findings suggest that abnormalities in matrix metalloproteinase (MMP) activity may be found in the cerebrospinal fluid (CSF) of patients with Creutzfeldt-Jakob disease (CJD). In this study of 16 subjects with CJD and 16 age-, and sex-matched controls, we determined the presence of MMP-2 and MMP-9 in their active and proenzyme forms, the relative levels of MMP-3 and four inhibitors of MMP activity (TIMP-1, TIMP-2, TIMP-3 and TIMP-4), and the concentration of 4-3-3 protein. The methodology used involved zymography and immunological techniques. The results indicate that, compared with controls, CJD patients have a significantly higher positive frequency of pro-MMP-9 and of the active form of MMP-2, along with significantly higher levels of TIMP-1 and TIMP-2, classical inhibitors of MMP-9 and MMP-2, respectively. We also found a positive correlation between 14-3-3 protein concentration and that of TIMP-1 and TIMP-2 levels (correlation coefficients of 0.793 and 0.798, respectively). These results suggest that abnormalities in MMP and TIMP profiles may be helpful in the biochemical characterisation of CJD.
MH Adult; Aged; Case-Control Studies; Creutzfeldt-Jakob Syndrome/cerebrospinal fluid/*enzymology; Female; Human; Male; Matrix Metalloproteinases/*cerebrospinal fluid; Middle Age; Support, Non-U.S. Gov't; Tissue Inhibitor of Metalloproteinases/*cerebrospinal fluid; Tyrosine 3-Monooxygenase/*cerebrospinal fluid
AD Department of Biochemistry and Molecular Biology, School of Chemical and Pharmaceutical Sciences, University of Chile, Santiago, Chile.
SP englisch
PO England