NR AOPQ
AU Aiken,J.M.; Johnson,C.; Johnson,J.; Vanderloo,J.; Clayton,M.; McKenzie,D.
TI Prion Protein Gene Heterogeneity in Wisconsin White-tailed Deer: Implications for CWD Susceptibility
QU International Conference - Prion diseases: from basic research to intervention concepts - TSE-Forum, 08.10.-10.10.2003, Gasteig, München - Poster session - DG-26
PT Konferenz-Poster
AB
Chronic Wasting Disease (CWD) is an expanding prion disease epidemic in wild and farm-raised deer and elk in the United States and Canada. From its initial occurrence in Colorado and Wyoming in the late 1960's, the disease has spread to free-ranging deer in New Mexico, Nebraska, Colorado, Wyoming, South Dakota, Saskatchewan, Wisconsin and Illinois.
Changes in the properties of the infectious agent (strains) are due to the combined influences of the original agents' properties and the new hosts' prion protein sequence. Since both of these factors play a role in disease propagation, it follows that the hosts' prion protein sequence (i) affects the susceptibility of that host to prion infection and (ii) affects the characteristics of the prions that result from the infection.
To determine whether specific Prnp genotypes were associated with CWD infection and transmission in deer in the Wisconsin CWD-affected area, Prnp was amplified and sequenced from deer that were either immunohistochemically positive (CWD-positive) or negative (CWD-negative) for CWD. Sequence analysis of Prnp from white-tailed deer in the CWD-affected area of Wisconsin identified four Prnp alleles, one of which, a glutamine to histidine change at codon 95, had not previously been identified in white-tailed deer. The predominant allele in the population encodes glutamine at codon 95, glycine at codon 96 and serine at codon 138 (QGS). Less abundant alleles were QSS, QGN and HGS. Comparison of the Prnp alleles from CWD-positive and CWD-negative deer indicate that 84-94% of the deer in the CWD-affected region have Prnp alleles associated with susceptibility to CWD infection. The QGS allele is over-represented and the QSS allele under-represented in the CWD-positive animals. These data suggest that selection of specific Prnp genotypes will not be an effective means of controlling CWD and that the possibility of CWD strains exists.
AD Judd M. Aiken, Chad Johnson, Jody Johnson, Joshua Vanderloo, Murray Clayton, Debbie McKenzie, University of Wisconsin-Madison, USA
SP englisch
PO Deutschland