NR AOPT
AU Arlotto,M.; Peoc'h,K.; Cazorla,M.; Hayer,M.; Windl,O.; Laplanche,J.L.; Lehmann,S.
TI Proteomic research of TSEs
QU International Conference - Prion diseases: from basic research to intervention concepts - TSE-Forum, 08.10.-10.10.2003, Gasteig, München - Poster session - DG-27
PT Konferenz-Poster
AB
The Proteome is defined as the expressed complement of a genome. Proteomics is the study of the properties of proteins (expression level, post-translational modifications, interactions etc.) on a large scale to obtain an integrated view of normal or pathological cellular processes or interactions at the protein level.
Our research programme aims to identify markers linked to TSEs using proteomic approaches. These markers will be evaluated in the diagnosis of the diseases as well as for the comprehension of their pathogenic processes.
We rely on two different proteomic technologies; i) a bidimensional (2D) electrophoresis approach, ii) a ProteinChip(r) System from Ciphergen Biosystems that uses patented SELDI (Surface-Enhanced Laser Desorption/Ionization) technology. We will first analyze samples from infected cellular models that have the advantage to represent genetically homogeneous systems. We will focus on comparing the "secretome" of the cells which will be represented by the range of proteins secreted for different periods of time in a conditioned medium. We believe that the analysis of this fraction could lead to the identification of a diffusible marker of TSEs. We will also compare the complete cell lysates of infected vs. control cells. The next step will be to analyze brain homogenates of infected and control animals before looking at samples from patients.
AD Marie Arlotto, Maxime Cazorla, Monique Hayer, Sylvain Lehmann, IGH du CNRS, UPR1142, Montpellier, France; Kattel Peoc'h, Jean-Louis Laplanche, Hopital Lariboisière, Paris, France; Otto Windl, Veterinary Laboratories Agency Weybridge, United Kingdom
SP englisch
PO Deutschland