NR AOQS
AU Brenig,B.; Schütz,E.; Urnovitz,H.
TI Isolation and characterization of humoral microvesicle encapsulated RNAs and their use in ante mortem diagnosis of BSE
QU International Conference - Prion diseases: from basic research to intervention concepts - TSE-Forum, 08.10.-10.10.2003, Gasteig, München - Oral sessions OS-31
PT Konferenz-Vortrag
AB Bovine Spongiform Encephalopathy (BSE) is a progressive neurodegenerative disease in cattle. The presence of BSE cattle in the food supply presents a public health concern to humans. Current tests for BSE are performed only at necropsy and therefore limit efforts for eliminating BSE animals from living herds. We have isolated and characterized humoral RNAs that differ between normal and diseased cattle. The detection of abnormal RNA profiles in cattle can be used for ante mortem diagnosis of BSE. RNA has already been shown to be suitable as surrogate marker for underlying chronic illnesses in humans and animals. Cattle with abnormal RNA patterns can be considered at high risk for BSE. RNA is present in the liquid fraction of blood samples from both healthy and diseased cattle. The RNA is found in the blood fraction that contains primarily microvesicles. RNA profiles have been shown to differ in humans with multiple myeloma and mysterious neurologic syndromes such as Gulf War Syndrome and Chronic Fatigue Syndrome. Based on our findings, a surrogate marker living test for BSE was developed that detects extracted RNA from bovine sera in a real time PCR test. The reaction pattern of the test sample is compared to healthy and BSE control samples. Samples that are more than three standard deviations above the normal healthy controls are considered reactive.
AD B. Brenig, Institute of Veterinary Medicine, University of Göttingen, Germany; E. Schütz, H. Urnovitz, Chronix Biomedical GmbH, Germany
SP englisch
PO Deutschland