NR AOSJ
AU Feldmann,G.; Bodemer,M.; Körtner,K.; Poser,S.; Zerr,I.
TI Levels of cellular prion protein (PrPc) in the cerebrospinal fluid in sCJD
QU International Conference - Prion diseases: from basic research to intervention concepts - TSE-Forum, 08.10.-10.10.2003, Gasteig, München - Poster session - BR-80
PT Konferenz-Poster
AB
Prion diseases have been recognized in many mammalian species and are characterized by the accumulation in the nervous system of the protease resistent isoform (PrPsc) of a host-coded membrane glycoprotein (PrPc). The role of the pathological isoform of the prion protein in the pathogenesis of prion diseases has been analyzed in detail, but the normal cellular function of PrPc still remains enigmatic. We thereby used an ELISA-based methodology to evaluate total PrPc levels in cerebrospinal fluid (CSF) from patients with neuropathological confirmed and probable CJD and controls with various other dementia.
In total, we analyzed 107 CSF samples. We measured the PrPc levels in CSF using the Platelia Detection Kit from BioRad. As a standard we used a recombinant prion protein (Prionics). The average concentration of PrPc in CSF from CJD patients was 24.6 ng/ml with a median of 24 ng/ml and a variation between 7 and 46.7 ng/ml. The comparison measurement between CSF from patients with AD and controls showed significant differences. The CSF from AD patients showed lower values than the controls.
To evaluate the diagnostic feasibility of total PrPc concentration further investigations are necessary. The next step will be the comparison between PrPc concentration with different other surrogat marker. It is also of interest to evaluate these data regarding the polymorphism of the prion protein gene.
This work was supported by the Bundesministerium für Bildung und Forschung (0312720)
AD G. Feldmann, M. Bodemer, K. Körtner, S. Poser, I. Zerr, University Hospital of Göttingen, Department of Neurology, Germany
SP englisch
PO Deutschland