NR AOSK
AU Fernandez Garcia,A.; Heindl,P.; Voigt,H.; Büttner,M.; Butz,P.; Tauscher,B.; Pfaff,E.
TI High pressure as a tool for investigating prion proteins
QU International Conference - Prion diseases: from basic research to intervention concepts - TSE-Forum, 08.10.-10.10.2003, Gasteig, München - Poster session - BR-59
PT Konferenz-Poster
AB Despite the typical stability of ß-sheet structures of proteins to high pressure treatments, the loss of resistance to proteolytic digestion of infectious hamster prions is remarkable after application of only several hundred MPa (500 up to 1000 MPa), following results obtained by Western-blots. Furthermore, a satisfactory agreement between delayed infectivity after intracerebral inoculation of pressurised pathological materials and the related sensitivity to proteinase K is proving prions to be extremely pressure sensitive, and therefore one of the most fascinating subjects of study in this field at present. Hence our results are revealing pressure to be on the one hand a successful tool to study prion misfolding with a potential application in understanding the transmission of amyloid diseases, but also a means to produce safe high-value products at temperatures below heat sterilisation parameters. This paper will address inactivation kinetics of pathological prions under pressure, and the complete results of bioassays in hamsters, which are currently being analysed. Furthermore, detailed studies on isolated fibrils will clarify whether the effects of pressure are directed to the cross-ß sheet structure assembling into amyloid fibrils of pathological prions.
AD Avelina Fernandez, Philipp Heindl, Peter Butz, Bernhard Tauscher, Federal Research Centre for Nutrition (Karlsruhe, Germany); Heiner Voigt, Matthias Büttner, Eberhard Pfaff, Federal Research Centre for Virus Diseases of Animals (Tübingen, Germany)
SP englisch
PO Deutschland