NR AOSM
AU Fichet,G.; Comoy,E.E.; Duval,C.; Antloga,K.; Bochot,C.; Battioni,P.; Mansuy,D.; McDonnell,G.; Deslys,J.P.
TI Disinfection of prion contaminated surfaces : from theory to practice.
QU International Conference - Prion diseases: from basic research to intervention concepts - TSE-Forum, 08.10.-10.10.2003, Gasteig, München - Poster session - IV-29
PT Konferenz-Poster
AB Prions present high capacities of adsorption on surfaces, and iatrogenic cases of Creutzfeldt-Jakob disease (CJD) occurred as a consequence of inadequate decontamination of medical and surgical devices. One of the main features of prions is their high resistance to almost all classical decontamination methods. Effective treatments are drastic and often present physical or chemical incompatibilities with surface materials. Moreover, the majority of studies have been conducted in solutions and many findings may not be applicable to prions adsorbed on solid surfaces. Indeed, reports on the use of fixed tissues or dry heat experiments give different results from those focusing on hydrated tissues. From prevoius studies with steel wires, we have developed and validated a protocol of contaminatination of similar wires adapted to the hamster model infected with the scrapie strain 263K. In this model, developed to obtain a homogeneously contaminated surface, we are able to explore a range of contamination levels within a range of 6 logs on a 2.5 mm2 surface of wire. The wires are placed intracerebrally to measure the levels of infectivity. We have also defined protocols to explore the possibilities of decontamination of PrPres aggregated as SAF (scrapie associated fibrils). These protocols have been used to evaluate recommended decontamination protocols (autoclaving, sodium hydroxide and sodium hypochlorite). We have also evaluated original methods including the use of porphyrins as catalysts, which allow to reduce the amount of oxidizers such as sodium hypochloride needed for decontamination. Our newly developed methods should allow to avoid the numerous biases which can occur when detecting limited contamination of solid surfaces. They will be discussed versus previous classical disinfection studies on prions in order to highlight the impact of the matrix of the contaminated surface and to draw practical conclusions.
AD Guillaume Fichet, Emmanuel E. Comoy (comoy@dsvidf.cea.fr), Christelle Duval, Jean-Philippe Deslys, CEA/DSV/DRM/GIDTIP, Route du Panorama, 92265 Fontenay-aux-Roses, France.; Kathleen Antloga, Gerald McDonnell, Laboratoire de Chimie et de Biochimie, CNRS UMR 8601/Université Paris V, 45 Rue des Saints-Pères, 75270 Paris, France.; C. Bochot, P. Battioni, D. Mansuy, STERIS Ltd., Jays Close, Viables, Basingstoke, United Kingdom.
SP englisch
PO Deutschland