NR AOTZ
AU Herde,K.A.; Bergmann,M.; Bülte,M.; Leiser,R.; Wenisch,S.
TI Glial proteins as species-specific markers for the detection of central nervous system tissue in heat-treated meat products
QU International Conference - Prion diseases: from basic research to intervention concepts - TSE-Forum, 08.10.-10.10.2003, Gasteig, München - Poster session - BR-56
PT Konferenz-Poster
AB
The new variant of Creutzfeldt-Jakob disease in the course of the bovine spongiforme encephalopathy (BSE) epidemic has focused attention on the use of central nervous tissue in food. Banning of bovine risk material cannot be effectively controlled as procedures for species-specific detection are not available so far.
Glial specific proteins such as glial fibrillary acidic protein (GFAP), myelin basic protein (MBP), and oligodendrocyte specific protein (OSP) are known to occur predominantely in CNS. The aim of the study was to evaluate (1) whether these proteins are still detectable in heat treated meat products and (2) whether commercially available antibodies are able to discriminate different species. We performed routine immunohistochemistry (ABC) using monoclonal antibodies against GFAP, MBP, and OSP on paraffin sections of bovine, ovine and porcine spinal cord and cooked sausages with varying addition (0%; 8%; 20%) of bovine brain. Western blotting was performed using protein extracts of organ and sausage homogenates. Additionally, tissue specificity was tested on paraffin sections of bovine and porcine peripheral nerves and muscles.
Anti-GFAP-, and anti-MBP-antibodies detected the presence of only bovine brain within the brain-containing reference material and bovine spinal cord, but not within porcine spinal cord and peripheral nerves and muscles of both species indicating, that these antibodies react organ- as well as species-specific. 0% sausage was also negative suggesting, that protein destruction due to the heating procedure does not lead to false positive results. OSP-antibodies did not react with any tissue. The results of Western blot analysis confirmed the staining pattern of immunohistochemistry.
Our data show, that GFAP and MBP glial proteins can be used as specific markers for the detection of bovine central nervous tissue in meat products in the scope of official food control.
AD K.A. Herde, M. Bergmann, R. Leiser, Institute of Veterinary -Anatomie, -Histology, and -Embryology; Justus-Liebig-University Giessen, Germany; S. Wenisch, Experimental Trauma Surgery; Justus-Liebig-University Giessen, Germany; M. Bülte, Institute of Veterinary Food Science; Justus-Liebig-University Giessen, Germany
SP englisch
PO Deutschland