NR AOUC
AU Horrocks,C.L.; Wilkinson,A.E.; Green,R.B.; Ryder,S.J.
TI Comparison of PrP IHC Patterns in BSE and Scrapie Infected Mice
QU International Conference - Prion diseases: from basic research to intervention concepts - TSE-Forum, 08.10.-10.10.2003, Gasteig, München - Poster session - BR-06
PT Konferenz-Poster
AB
Bovine spongiform encephalopathy (BSE) and scrapie belong to a group of diseases called transmissible spongiform encephalopathies (TSE's). Lesion profiling in mouse transmission studies (Fraser and Dickinson, 1968) coupled with incubation period analyses have provided the dominant tools for differentiating prion strains. Using this technique BSE has produced a characteristic profile in RIII mice, apparently distinct from that of the numerous isolated scrapie strains. Several recent primary isolates of sheep scrapie have produced profiles that are similar in some respects to BSE so further means of discrimination have been investigated.
In this study we look at immunohistochemical analysis as a further tool to aid in the differentiation of BSE from scrapie. Brain tissue taken from a broad range of transmission studies carried out at the Veterinary Laboratories Agency have been used to compare the immunostaining patterns in histologically confirmed disease in inbred RIII and C57bl mouse strains. For each mouse strain, the type and the distribution of abnormal PrP have been recorded.
Accumulation of PrP in both BSE and scrapie challenged mice produced distinct immunostaining patterns. The primary sites for PrP deposition in BSE inoculated mice were the medulla oblongata and red nuclei. In scrapie inoculated mice PrP deposition was more widespread, involving the thalamus, cerebellar granule cell layer, hippocampus and mid-brain.
Brains of BSE and scrapie infected mice showed morphologically similar granular PrP deposits that were confined to the neuropil. These deposits differed greatly in the degree of intensity. Plaques were consistently observed in the brains of scrapie inoculated mice but rarely in BSE.
We conclude that PrP immunostaining analysis is an important addition to conventional lesion profiling techniques for distinguishing BSE from scrapie.
AD C.L. Horrocks, A.E. Wilkinson, R.B. Green, S.J. Ryder, Veterinary Laboratories Agency, UK
SP englisch
PO Deutschland