NR AOVO
AU Latawiec,D.; Sussman,J.; Nelken,P.; DeArmond,S.J.; Prusiner,S.B.; Legname,G.
TI Localization of prion and doppel protein ligands in mouse brain
QU International Conference - Prion diseases: from basic research to intervention concepts - TSE-Forum, 08.10.-10.10.2003, Gasteig, München - Poster session - BR-72
PT Konferenz-Poster
AB
We recently described the binding of mouse (Mo) prion protein (PrPc) and its paralog protein doppel (Dpl) to various regions of the mouse brain, using novel fusion proteins composed of either MoPrPc or MoDpl and the Fc g1 domain of immunoglobulin proteins. These molecules are denoted MoPrP-Fc and MoDpl-Fc, respectively (Legname et al. 2002). Histoblotting revealed that both MoPrP-Fc and MoDpl-Fc bound to many regions of the brain, but we observed restricted binding to granule cells in the cerebellum. These findings suggest that granule cell neurons might express a protein or protein complex that mediates PrPc function and Dpl-induced neurodegeneration (Moore et al. 2001). Because granule cells may not express PrPc, as demonstrated using recombinant Fab fragments that specifically recognize MoPrPc, it is unlikely that (i) MoPrP-Fc binds to itself, and that (ii) this binding reflects a ligand involved in the conversion of PrPc into PrPsc, the disease-causing isoform.
We will present the most recent results on the characterization of neuronal cells involved in the binding of MoPrP and MoDpl, including findings from immunochemical and immunohistochemical studies employing several Fc-fusion proteins.
References
Legname et al., Prion and doppel proteins bind to granule cells of the cerebellum. Proc Natl Acad Sci U S A. 2002 Dec 10;99(25):16285-90.
Moore et al., Doppel-induced cerebellar degeneration in transgenic mice. Proc Natl Acad Sci U S A. 2001 Dec 18
AD Diane Latawiec, Josh Sussman, Stephen J. DeArmond, Stanley B. Prusiner, Giuseppe Legname, Institute for Neurodegenerative Diseases/UCSF/USA; Peter Nelken, Stephen J. DeArmond, Department of Pathology/UCSF/USA; Giuseppe Legname, Stanley B. Prusiner, Department of Neurology/UCSF/USA; Stanley B. Prusiner, Departmets of Biochemistry and Biophysics/UCSF/USA
SP englisch
PO Deutschland