NR AOWK
AU Masuch,R.; Seidel,R.; Wolf,A.; Schuster,K.; Jankowsky,R.
TI A Novel Ante Mortem Test System For The Diagnosis Of Bovine Spongiform Encephalopathy Based On Direct FT-IR Measurements In Liquid Serum Samples
QU International Conference - Prion diseases: from basic research to intervention concepts - TSE-Forum, 08.10.-10.10.2003, Gasteig, München - Poster session - DG-37
PT Konferenz-Poster
AB A test system based on Fourier Transform Infrared Spectroscopy (FT-IR) combined with a novel flow cell technology (AquaSpec technology) was developed for ante mortem diagnosis of BSE and could be shown to detect BSE-infected animals with high accuracy. The AquaSpec technology allows the direct FT-IR measurement of liquid serum samples without pre-treatment of the samples. The test comprises two steps: I) recording of infrared spectra of the serum samples and II) computerised multivariate analysis of the infrared spectra by a proprietary data algorithm (pattern recognition using an artificial neural network). For setup of the data algorithm, spectra of 130 serum samples from terminally ill BSE-positive animals (VLA Weybridge, UK) and 260 serum samples from BSE-negative animals (130 samples from VLA Weybridge and 130 samples from Waldkraiburg abattoir, Germany) were recorded and used as input (training) of the artificial neural network. After setup of the data algorithm, the test was evaluated in a blinded study using previously unknown serum samples from 40 terminally ill BSE-positive animals and 40 BSE-negative samples from BSE-negative samples. All samples from BSE-positive animals and BSE-negative animals were determined accurately yielding a diagnostic sensitivity of 100% and a diagnostic specificity of 100% for the test system. Effects of the sample age and / or sample origin on the test result were excluded by internal validation of the data algorithm using serum samples of different age and origin. These results show for the first time that BSE can be accurately detected in serum samples by the FT-IR flow cell technology. A prototype of the test system was developed as a fully automated bench-top analyser. The testing protocol is very simple with a total required time from sampling to result of less than one hour. The test has a high potential for routine BSE testing and will be evaluated in a field study within short.
AD R. Masuch, A. Wolf, micro-biolytics GmbH, Freiburg, Germany; R. Seidel, DiaSpec GmbH, Freiburg, Germany; K. Schuster, R. Jankowsky, SCIL Diagnostics GmbH, D-82152 Martinsried
SP englisch
PO Deutschland