NR AOXG
AU Mutinelli,F.; Aufiero,G.M.; Vascellari,M.; Vaccari,G.; Agrimi,U.; Nonno,R.; Pozzato,N.; Magarotto,R.; Falcaro,C.; Basilicata,L.
TI Western Blotting and Immunohistochemistry Patterns in Nervous and Lymphoid Tissues of Susceptible PrP Genotype Massese Breed Sheep with Subclinical Scrapie
QU International Conference - Prion diseases: from basic research to intervention concepts - TSE-Forum, 08.10.-10.10.2003, Gasteig, München - Poster session - PG-16
PT Konferenz-Poster
AB In 2002, two scrapie outbreaks were detected in Massese breed sheep flocks in Veneto Region (northern Italy). Due to the importance of the Massese breed in Italian sheep population, selective culling of sheep with susceptible PrP genotype (680 out of 1,898) has been applied, as an alternative to the enforcement of stamping out measures. All culled sheep were tested with the Prionics Check-Western rapid test using two monoclonal antibodies (6H4; P4), giving 22 positive animals. PrP genotype was ARQ/ARQ (18), ARQ/VRQ (2) and ARQ/AHQ (2). The P4 antibody revealed some positive cases not detected by the 6H4 antibody. This suggests that the P4 antibody could have a higher sensitivity than 6H4. All cases positive to the rapid test performed with the P4, were positive on the obex by immunohistochemistry (mAb F99/97.6.1). In addition, the intensity of the positive signal detected on Western-Blotting agreed with the accumulation of PrPsc as detected immunohistochemically. The tonsil, the retropharingeal lymph node and the third eyelid of the sheep positive to the rapid test, were examined on immunohistochemistry using the monoclonal antibody F99/97.6.1. All the lymphoid organs investigated were positive in 15 out of 22 cases. In 4/22 cases the retropharingeal lymph node and the tonsil were positive, while the third eyelid resulted negative. In 3/22 cases all the lymphoid organs investigated were negative. These findings show that the scrapie agent may reach the brain without detectable PrPsc accumulation in lymphoid organs. No evident association between PrPsc accumulation in lymphoid organs and PrP genotype was observed. Finally, we performed immunohistochemistry using antibodies recognizing different epitopes (P4 and 34C9), in order to better characterise the pattern of positivity and PrPsc distribution in lymphoid tissues in the Massese breed sheep.
AD Franco Mutinelli, Giovanni M. Aufiero, Marta Vascellari, Roberto Magarotto, Christian Falcaro, Lara Basilicata, Istituto Zooprofilattico Sperimentale delle Venezie, Italy; Gabriele Vaccari, Umberto Agrimi, Romolo Nonno, Istituto Superiore di Sanità, Italy; Nicola Pozzato, Centro Regionale di Epidemiologia Veterinaria, Italy
SP englisch
PO Deutschland