NR AOXO
AU Oidtmann,B.; Hoffmann,R.W.; Rivera-Milla,E.; Stuermer,C.A.O.; Malaga-Trillo,E.; Torgensen,J.; Syed,M.; Holtkamp,N.; Hartmann,C.; Baier,M.
TI Prion proteins in fish: Identification of duplicated genes for homologues to tetrapod prion proteins in teleost fish
QU International Conference - Prion diseases: from basic research to intervention concepts - TSE-Forum, 08.10.-10.10.2003, Gasteig, München - Poster session - DG-50
PT Konferenz-Poster
AB
We have identified cDNAs coding for homologues to tetrapod prion proteins (PrP) in Atlantic salmon, Rainbow trout, common carp and duplicated PrP cDNAs in Japanese pufferfish, zebrafish, and stickleback. Besides significant sequence homologies the fish PrPs display characteristic structural features in common with tetrapod PrPs.
We also identified a further group of PrP related sequences in Fugu, zebrafish and carp, called PrP-related proteins (PrP-rel): the sequences show very high homology with the mammalian PrPs in the hydrophobic region, but lack other important features, such as the two cystein residues or a repeat region. In 2002, Suzuki et al. reported a cDNA coding for a putative prion-like protein in Japanese pufferfish, which is also lacking some characteristic key features of PrPs, but has a well conserved hydrophobic region.
Analysis of the position of those genes within the Fugu genome showed that, PrP-a and PrP-rel are located in immediate neighbourhood on scaffold 96, whereas PrP-b and prion-like are found in close vicinity on scaffold 155, suggesting teleost-specific genome duplications.
Furthermore, Fugu PrP-b coding region is located close to the Fugu homologues of RASSF2 and SLC231A on scaffold 155. In the human genome RASSF2 and SLC231A are located together with PrP and doppel on chromosome 20p13, which suggests a common ancestor for PrP-b and the mammalian PrPs.
Expression patterns of salmon and carp PrPs were analysed by Northern blot, showing in salmon the most prominent expression in the brain. However in carp the expression level of PrP was at equally high levels in muscle tissue compared to brain tissue.
AD Birgit Oidtmann, Rudolf W. Hoffmann, Institute of Zoology, Fish Biology and Fish Diseases, University of Munich, Germany; Michael Baier, Robert-Koch-Institute, Berlin, Germany; Eric Rivera-Milla, Claudia A.O. Stuermer, Edward Malaga-Trillo, Department of Biology, University of Konstanz, Germany; Jakob Torgensen, Mohasina Syed, Department of Morphology, Genetics and Aquatic Biology, Norwegian School of Veterinary Science; Nikola Holtkamp, Christian Hartmann, Institute of Neuropathology, Humboldt University Berlin, Germany
SP englisch
PO Deutschland