NR AOXS
AU Paspaltsis,I.; Lagoudaki,R.; Kalpatsinidis,A.; Grigoriadis,N.; Poulios,I.; Sklaviadis,T.K.
TI Application of Fenton Photocatalysis to Inactivation of Prions
QU International Conference - Prion diseases: from basic research to intervention concepts - TSE-Forum, 08.10.-10.10.2003, Gasteig, München - Poster session - BR-109
PT Konferenz-Poster
AB
In 1977 the first instance of transmission of the TSE pathogen through neurosurgical instruments was reported. At the time information about TSE pathogens was minimal, as was any knowledge of their nature. Today our understanding of the prion diseases is vastly greater, supported by a large number of publications and a wealth of experimental data. Despite this improved understanding, however, inactivation of prions remains problematic due to their resistance to conventional methods of decontamination. Experimentally effective methods are inadequate for routine use in daily practice for several reasons. In some cases their usefulness is limited to only a few applications in the broad range of fields where prions may occur. In others they are impractical or cost-ineffective on a large scale. Thus, iatrogenic cases of TSE disease remain a problem. As recently as the year 2000 in Australia, for instance, there was a report that "nine hospital patients may have been exposed to CJD. "
In this report we present data supporting the use of the homogenous photocatalytic oxidation method (Photo-Fenton reagent) for prion inactivation. The potential of Fe3+ / H2O2 to oxidize organic pollutants is already known. It utilizes the light energy of the UV-A visible spectrum to produce free OH radicals, which serve as oxidizing agents. When the reaction goes to completion the final products are CO2 and H2O. Degradation of proteins such serum albumin, recombinant prion protein and the proteins of brain homogenates is demonstrated. Furthermore after Photo-Fenton treatment of infected brain homogenates (3mg brain equivalents) according to our methodology, the PrPsc content of the homogenate is no longer detectable by western blot. The potential of Fenton photocatalysis to inactivate PrPsc will be tested in a bioassay on golden Syrian hamsters.
AD I. Paspaltsis, T. Sklaviadis, Laboratory of Pharmacology, School of Pharmaceutical Sciences, Aristotle University of Thessaloniki, Thessaloniki 540 06, Greece; R. Lagoudaki, A. Kalpatsinidis, N. Grigoriadis, B' Dept. of Neurology and Lab of experimental Neurology, AHEPA University hospital, 54636 Thessaloniki, Greece; I. Poulios, Laboratory of Physical Chemistry, Dept. of Chemistry, Aristotle University of Thessaloniki, Thessaloniki 540 06, Greece
SP englisch
PO Deutschland