NR AOXV
AU Phillips,K.E.; Johnson,C.J.; McKenzie,D.I.; Aiken,J.M.; Pedersen,J.A.
TI Adsorption of PrPsc to Well-Defined Soil Constitutents
QU International Conference - Prion diseases: from basic research to intervention concepts - TSE-Forum, 08.10.-10.10.2003, Gasteig, München - Poster session - BR-62
PT Konferenz-Poster
AB
Compelling circumstantial evidence suggests that soil may serve as an environmental reservoir for the infectious agent in transmissible spongiform encephalopathies (TSEs) of ovids and cervids. Evidence in support of this hypothesis includes observations that healthy sheep and elk have contracted TSEs upon introduction into fields and enclosures previously occupied by infected animals, as well as the retention of residual infectivity in garden soil after PrPsc-infected hamster brain material had been interred for 3 years. Preservation of TSE agents in soil is probably due to the sorption to specific soil components. Soils vary considerably in mineral and organic matter content, and the degree of prion preservation likely depends on soil composition. The extent of sorption is typically controlled by surface-reactive soil particles including clay minerals, aluminum and iron (hydr)oxides, and humic substances.
We are investigating the association of PrPsc with well-defined soil components and examining the degree to which sorption influences the stability of the infectious agent in the environment. Batch sorption experiments were conducted with smectitic clay and quartz sand to determine the extent of adsorption to these minerals. Free and mineral surface-adsorbed PrPsc fractions were separated by low-speed centrifugation through a sucrose cushion, and the amount of PrPsc adsorbed determined by Western blot analysis. Desorption experiments were conducted to account for all the added PrPsc. Initial results indicate that the PrPsc-adsorption capacity of clay minerals substantially exceeds that of quartz sand. These results have important implications for the mobility and bioavailability of the infectious agent in soil environments.
AD Kristen E. Phillips, Joel A. Pedersen, Molecular and Environmental Toxicology Center, University of Wisconsin - Madison, USA; Christopher J. Johnson, Program in Cellular and Molecular Biology, University of Wisconsin- Madison; Christopher J. Johnson, Debbie I. McKenzie, Judd M. Aiken, Animal Health and Biomedical Sciences, University of Wisconsin - Madison; Joel A. Pedersen, Department of Soil Science, University of Wisconsin-Madison
SP englisch
PO Deutschland