NR AOYT
AU Satoh,K.; Shirabe,S.; Tsujino,A.; Nishiura,Y.; Eguchi,K.; Satoh,A.; Tsujihata,M.; Matsuo,H.
TI Role of phospholylated tau/total tau ratio, 14-3-3 protein in CSF and diffusion weighted MRI in diagnosis of Creutzfeldt-Jakob disease
QU International Conference - Prion diseases: from basic research to intervention concepts - TSE-Forum, 08.10.-10.10.2003, Gasteig, München - Poster session - DG-07
PT Konferenz-Poster
AB
Attempts to develop the therapeuticc agents of Creutzfeldt-Jakob disease (CJD) including anti-infectious agents, immunomodulating agents chemically interacting with prion protein are in progress. As CJD is a rapidly progressive disease, it is urgent to establish the diagnostic tools of CJD in early stage.
We investigated the role of diffusion-weighted MR images (DWI), 14-3-3 protein, total tau protein and phospholyrated tau protein in cerebrospinal fluid (CSF). We tested CSF derived from 100 patients: CJD (n=10), Alzheimer-type dementia (ATD) (n=72), Vascular dementia (n=7), Pick disease (n=2), Parkinson disease (n=3), corticobasal degeneration (n=2), Huntington disease (n=1), frontotemporal dementia (n=1), progressive supranuclear palsy (n=3), ALS (n=3), Hysteria (n=2), and normal control (n=2). Ten cases of CJD included 4 ?gdefinite?h cases (1 case of M232R, 2 cases of MM2-cortical form, 1case of MM1) and 6 ?gprobable?h cases. All ten CJD patients showed positive 14-3-3 protein and elevated total tau protein (>1000 pg/ml).
In two ATD patients, we identified positive 14-3-3 protein and elevated tau protein as high as those of CJD. The major difference between to clearly distinguish ATD and CJD was ratio of phospho-tau/ total tau protein of CSF.
We also present 14-3-3 protein and elevated total tau protein from CSF of one CJD patient detected through his whole clinical course.
Recent reports suggested that high intensities at cortex and basal ganglia detected by DWI are useful for diagnosis of CJD in early stage of its clinical course. However, in one case of sporadic CJD, we could detect elevated total tau protein in CSF two weeks prior to detection of the typical changes of DWI in early stage of CJD. In this case, 14-3-3 protein in CSF was only equivocally positive by using CSF obtained the same time. We suggest that measurement of total tau protein of CSF could be more useful for diagnosis of CJD in early stage.
AD Katsuya Satoh, Susumu Shirabe, Akira Tsujino, Yoshihiro Nishiura, Katsumi Eguchi, Nagasaki University; Akira Satoh, Mitsuhiro Tsujihata, Nagasaki Kita Hospital; Hidenori Matsuo, Kawatana Hospital
SP englisch
PO Deutschland