NR AOYU
AU Sauer,M.J.; Tang,H.R.; Ryder,S.; Lindon,J.C.
TI Potential of Blood Serum Metabonomics to Detect Scrapie in Sheep
QU International Conference - Prion diseases: from basic research to intervention concepts - TSE-Forum, 08.10.-10.10.2003, Gasteig, München - Poster session - DG-17
PT Konferenz-Poster
AB
Investigating the occurrence and nature of significant changes in metabolite profile during the progression of scrapie presents the prospect of identifying surrogate markers of disease and of advancing understanding of associated pathology.
To this end a pilot metabonomic study of sheep blood serum has been conducted using nuclear magnetic resonance spectroscopy (NMR). Samples (n = 20 in each case) were collected from uninfected sheep (control), and from scrapie infected sheep showing clear signs (clinical) or no signs (pre-clinical) of disease. Water-suppressed 1H NMR spectra were obtained from sera and data reduced for chemometric analysis. Principal components (PC) plots showed that the control and clinical groups could be essentially completely separated from each other. A lesser degree of separation was also apparent between the control and pre-clinical disease samples (the disease was confirmed in all cases at p.m.). Interrogation of the PC loadings reveal which spectral regions are responsible for the clustering, and the identification of the substances giving rise to NMR peaks in those regions is ongoing. This will enable a preliminary understanding of the type of biomarker responsible for the separation. A full structural determination of the biomarker combination will, however, require considerable application of 2-dimensional NMR spectroscopy, mass spectrometry, and probably HPLC-NMR-MS.
This preliminary study demonstrates the ability to identify animals in clinical and possibly preclinical stages of scrapie infection through the analysis of readily accessible body fluids and holds promise for future diagnostic applications.
AD Maurice J. Sauer, TSE Molecular Biology Department, Veterinary Laboratories Agency, Weybridge, UK; Huiru R. Tang, Biological Chemistry, Biomedical Sciences Division, Imperial College, London, UK; Steven Ryder, Pathology Department, Veterinary Laboratories Agency, Weybridge, UK; John C. Lindon, Biological Chemistry, Biomedical Sciences Division, Imperial College, London, UK
SP englisch
PO Deutschland