NR AOZA
AU Schönenbrücher,H.; Abdulmawjood,A.; Bülte,M.
TI Real time RT-PCR for detection of bovine-specific central nervous system tissues as BSE risk material in meat and meat products
QU International Conference - Prion diseases: from basic research to intervention concepts - TSE-Forum, 08.10.-10.10.2003, Gasteig, München - Poster session - BR-08
PT Konferenz-Poster
AB
With regard to an efficient preventive health protection of consumer's European legislation prohibited specified bovine offals (SBOs), e. g. central nervous system (CNS) tissues, from food chain. The identification of bovine-specific CNS tissues is an essential prerequisite for effective control of a potential source of human bovine spongiform encephalopathy (=new variant of Creutzfeldt-Jakob disease). Till now several chemical and immunological methods for detection of tissues of the CNS in meat products have been published to be used in food safety control.
The aim of the own project was to identify the bovine-specific BSE risk material in meat and meat products. For this reason, a novel molecular method based on real-time reverse transcriptase (RT)-PCR was developed. A specific small region of Glial fibrillary acidic protein (GFAP)-mRNA has been selected and employed to reach the goal of detection of illegal use of bovine CNS in raw as well as in heat-treated meat and meat products. With this system 0.01 % of bovine CNS tissues could be detected even in heat treated sausages, while porcine CNS tissue showed no signal.
* This work is a part of a project including three German veterinarian institutes at the Justus-Liebig-University Giessen and is founded by the Federal Ministry of Consumers Protection, Food and Agriculture (AZ.: 01HS022/1).
AD Holger Schönenbrücher, Amir Abdulmawjood, Michael Bülte, Institute of Veterinary Food Science, Justus-Liebig-University Giessen, Germany
SP englisch
PO Deutschland