NR AOZI
AU Spiropoulos,J.; Casalone,C.; Caramelli,M.; Simmons,M.M.
TI Correlation between PrPc genotype and PrPsc deposition in the ovine obex
QU International Conference - Prion diseases: from basic research to intervention concepts - TSE-Forum, 08.10.-10.10.2003, Gasteig, München - Poster session - PG-13
PT Konferenz-Poster
AB
Detection of PrPsc in sheep brains using immunohistochemistry can reveal specific patterns of PrP deposition. The aim of the current study was to investigate if the occurrence and anatomical location of these patterns were associated with PrP genotype.
PrP was detected using either the monoclonal antibody R145 or the polyclonal R486 on the obex of sheep from scrapie positive cases. Nine different patterns of PrP deposition were identified using published criteria (1, 2). The same PrP pattern could be identified using either antibody although improved stain intensity was apparent with R145. For each nucleus the type of PrP deposition was noted, graded according to the intensity of the staining and the predominant pattern(s) were recorded. We compared animals from 34 flocks: 31 from the UK and 3 from Italy. At least 35 animals were analysed per genotype (ARQ/ARQ, ARQ/VRQ and VRQ/VRQ). Specific PrP deposition patterns in the obex were found to consistently segregate with genotype.
Each homozygous genotype had distinct patterns associated with it. Heterozygotes displayed different patterns which were either unique to the genotype or showed some similarity to the patterns of the related homozygote. In these animals certain alleles appeared to have a dominant effect on the PrP pattern. Differences in the PrP patterns within the same genotype could not be explained by breed or flock differences so we are tempted to speculate that differences in PrP pattern distribution may be attributable to different PrP strains.
References:
1. Ryder S. J. et. al. Vet. Rec. 148: 7-13(2001);
2. Gonzalez L. et. al. J. Comp. Path. 126: 17-29 (2002).
AD J. Spiropoulos, M.M. Simmons, Veterinary Laboratories Agency, UK; C. Casalone, M. Caramelli, CEA-Centro Encefalopatie Animali, Italy
SP englisch
PO Deutschland