NR APAB
AU Vidal,E.; Ordonez,M.; Vela,J.M.; Pumarola,M.
TI Study of the olfactory pathway and related structures of 19 BSE naturally affected cows
QU International Conference - Prion diseases: from basic research to intervention concepts - TSE-Forum, 08.10.-10.10.2003, Gasteig, München - Poster session - PG-26
PT Konferenz-Poster
AB
Cellular prion protein (PrPc) distribution studies performed in hamsters demonstrate larger amounts of PrPc in the olfactory nerves in comparison to other white matter areas, this is due to the fact that those axons are continuously growing. Recently Zanusso G. et al. (2003) have recently described the presence of pathological resistant prion protein (PrPres) at the olfactory mucosa of humans affected by CJD. Both authors hypothesize about the possibility of an involvement of the olfactory pathway in the pathogenesis of TSEs.
Each time a positive case is diagnosed in Catalonia (Spain) our laboratory (PRIOCAT lab, CReSA Foundation) confirms the diagnosis and collects samples from the skull (including the whole brain and other cranial structures). Up to date, 19 BSE naturally affected cows have been detected by routine screening through the abattoir surveillance program. A biochemical (immunoblot) and immunohistochemical study of the PrPres distribution amongst the different brain areas has been performed.
The biochemical study revealed that the presence of PrPres was more abundant at the brain stem level in all cases, yet, in some instances, other brain structures were also involved. About a half of the animals studied (10/19) had positivity to PrPres at the olfactory bulbs, in all these cases the neocortex was also positive as well as the paleocortex (piriform lobe) and the archicortex (hippocampus). In the cases where the olfactory bulb was negative (9/19) the different cortical lobes where generally negative (8/9).
The olfactory mucosa was studied in three cases yielding always a negative result at both immunoblotting and immunohistochemical studies.
In this poster the results obtained by both techniques are described and the involvement of the olfactory pathway and its related structures (Olfactory mucosa, bulbs and peduncles, piriform lobe and hippocampus) in the pathogenesis of BSE is discussed.
IN Die Autoren haben bei 19 der bisherigen BSE-Fälle in Spanien die Verteilung des Protease-resistenten Prionproteins untersucht. Die höchsten Konzentrationen fanden sie in allen Fällen im Stammhirn. Bei 10 der 19 Fälle konnte man es auch in den Bulbi olfaktorii, im Neokortex (junger Teil der Großhirnrinde), im Paleokortex (alter Teil der Großhirnrinde wie Hippocampus), Lobus piriformis und im Hippocampus nachweisen. Wo die Bulbi olfaktorii PrPsc-negativ waren (9/19), dann fand man fast immer (8/9) auch in den verschiedenen Großhirnlappen nichts. In keinem von 3 daraufhin untersuchten Fällen fand man PrPsc in der Riechbahn von den Riechschleimhäuten bis zum Lobus piriformis.
AD Enric Vidal, Montserrat Ordonez, Marti Pumarola, PRIOCAT Laboratory, CReSA Foundation, Spain; Jose Miguel Vela, Marti Pumarola, Animal Medicine and Surgery Department, Veterinary Faculty, Autonomous University of Barcelona, Spain
SP englisch
PO Deutschland
ZF kritische Zusammenfassung von Roland Heynkes