NR APHU
AU Head,M.W.; Ritchie,D.; McLoughlin,V.; Ironside,J.W.
TI Investigation of PrPres in dental tissues in variant CJD
QU British Dental Journal 2003 Sep 27; 195(6): 339-43; discussion 331
PT journal article
AB OBJECTIVES: To study the distribution of disease-associated prion protein (PrP) in oral and dental tissues in variant CJD. DESIGN: Prospective single centre autopsy based study. SETTING: Within the National CJD Surveillance Unit, UK, 2000-2002. MATERIALS AND METHODS: Patients with suspected variant CJD undergoing autopsy where permission to remove tissues for research purposes had been obtained from the relatives. Fixed and frozen autopsy tissues from the brain, trigeminal ganglion, alveolar nerve, dental pulp, gingiva, salivary gland, tongue and tonsils were studied by Western blot, PET blot and immunocytochemistry to detect disease-associated PrP. RESULTS: Disease-associated PrP was only detected in the brain, trigeminal ganglia and tonsils. CONCLUSIONS: The failure to detect disease-associated PrP in most dental and oral tissues will help inform ongoing risk assessments for dental surgery in relation to the possible iatrogenic transmission of variant CJD via dental instruments.
MH Adult; Aged; Brain Chemistry; Creutzfeldt-Jakob Syndrome/prevention & control/*transmission; *Dental Care for Chronically Ill; Dental Instruments; Equipment Contamination; Female; Human; Immunoblotting; Immunoenzyme Techniques; Male; Middle Aged; PrPsc Proteins/*analysis; Prospective Studies; Support, Non-U.S. Gov't; Tissue Distribution; Tonsil/chemistry; Trigeminal Ganglion/chemistry
AD M.W.Head, Senior Research Fellow, D.Ritchie, Research Assistant, V.McLoughlin, Laboratory Technician, J.W.Ironside (james.ironside@ed.ac.uk), Director, National Creutzfeldt-Jakob Disease Surveillance Unit, Division of Pathology, School of Clinical and Molecular Medicine, University of Edinburgh, Western General Hospital, Edinburgh EH4 2XU
SP englisch
PO England