NR APIL
AU Johnson,C.; Johnson,J.; Clayton,M.; McKenzie,D.; Aiken,J.M.
TI Prion protein gene heterogeneity in free-ranging white-tailed deer within the chronic wasting disease affected region of Wisconsin
QU Journal of Wildlife Diseases 2003 Jul; 39(3): 576-81
IA http://www.jwildlifedis.org/cgi/reprint/39/3/576
PT journal article
AB Chronic wasting disease (CWD) was first identified in Wisconsin (USA) in whitetailed deer (Odocoileus virginianus) in February 2002. To determine if prion protein gene (Prnp) allelic variability was associated with CWD in white-tailed deer from Wisconsin, we sequenced Prnp from 26 CWD-positive and 100 CWD-negative deer. Sequence analysis of Prnp suggests that at least 86-96% of the white-tailed deer in this region have Prnp allelic combinations that will support CWD infection. Four Prnp alleles were identified in the deer population, one of which, resulting in a glutamine to histidine change at codon 95, has not been previously reported. The predominant allele in the population encodes for glutamine at codon 95, glycine at codon 96, and serine at codon 138 (QGS). Less abundant alleles encoded QSS, QGN, and HGS at the three variable positions. Comparison of CWD-positive with CWD-negative deer suggested a trend towards an over-representation of the QGS allele and an under-representation of the QSS allele.
MH Alleles; Animals; Animals, Wild; DNA, Viral/analysis; Deer/*virology; Gene Amplification; Genetic Predisposition to Disease; Prions/analysis/*genetics; Support, Non-U.S. Gov't; *Variation (Genetics); Wasting Disease, Chronic/*genetics; Wisconsin
AD Chad Johnson, Jody Johnson, Debbie McKenzie, Judd Aiken (aiken@svm.vetmed.wisc.edu), Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison, Wisconsin 53706, USA; Murray Clayton, Departments of Statistics and Plant Pathology, University of Wisconsin, Madison, Wisconsin 53706, USA
SP englisch
PO USA