NR APLA
AU Prinz,M.; Heikenwalder,M.; Junt,T.; Schwarz,P.; Glatzel,M.; Heppner,F.L.; Fu,Y.X.; Lipp,M.; Aguzzi,A.
TI Positioning of follicular dendritic cells within the spleen controls prion neuroinvasion
QU Nature 2003 Oct 30; 425(6961): 957-62
PT journal article
AB Peripheral infection is the natural route of transmission in most prion diseases. Peripheral prion infection is followed by rapid prion replication in lymphoid organs, neuroinvasion and progressive neurological disease. Both immune cells and nerves are involved in pathogenesis, but the mechanisms of prion transfer from the immune to the nervous system are unknown. Here we show that ablation of the chemokine receptor CXCR5 juxtaposes follicular dendritic cells (FDCs) to major splenic nerves, and accelerates the transfer of intraperitoneally administered prions into the spinal cord. Neuroinvasion velocity correlated exclusively with the relative locations of FDCs and nerves: transfer of CXCR5-/- bone marrow to wild-type mice induced perineural FDCs and enhanced neuroinvasion, whereas reciprocal transfer to CXCR5-/- mice abolished them and restored normal efficiency of neuroinvasion. Suppression of lymphotoxin signalling depleted FDCs, abolished splenic infectivity, and suppressed acceleration of pathogenesis in CXCR5-/- mice. This suggests that prion neuroimmune transition occurs between FDCs and sympathetic nerves, and relative positioning of FDCs and nerves controls the efficiency of peripheral prion infection.
MH Animals; Bone Marrow Transplantation; Chimera/immunology/metabolism; Dendritic Cells/*immunology; Gene Deletion; Kinetics; Mice; Mice, Knockout; Prions/immunology/*metabolism; Receptors, Cytokine/genetics/metabolism; Receptors, Tumor Necrosis Factor/antagonists & inhibitors/metabolism; Scrapie/*immunology/metabolism/*pathology; Signal Transduction; Spinal Cord/metabolism/pathology; Spleen/*immunology/innervation/metabolism/pathology; Support, Non-U.S. Gov't; Sympathetic Nervous System/immunology/metabolism/pathology
AD Institute of Neuropathology, University Hospital of Zürich, Schmelzbergstrasse 12, CH-8091 Zürich, Switzerland.
SP englisch
PO England