NR APTW
AU Archer,F.; Bachelin,C.; Andreoletti,O.; Besnard,N.; Perrot,G.; Langevin,C.; Le Dur,A.; Vilette,D.; Baron-van Evercooren,A.; Vilotte,J.L.; Laude,H.
TI Cultured peripheral neuroglial cells are highly permissive to sheep prion infection
QU Journal of Virology 2004 Jan; 78(1): 482-90
IA http://www.pubmedcentral.gov/picrender.fcgi?artid=303391&blobtype=pdf
PT evaluation studies; journal article
AB Transmissible spongiform encephalopathies arise as a consequence of infection of the central nervous system (CNS) by prions. Spreading of the infectious agent through the peripheral nervous system (PNS) may represent a crucial step toward CNS neuroinvasion, but the modalities of this process have yet to be clarified. Here we provide further evidence that PNS glial cells are likely targets for infection by prions. Glial cell clones originating from dorsal root ganglia of transgenic mice expressing ovine PrP (tgOv) and simian virus 40 T antigen were found to be readily infectible by sheep scrapie agent. This led us to establish two stable cell lines that exhibited features of Schwann cells. These cells were shown to sustain an efficient and stable replication of sheep prion based on the high level of accumulation of abnormal PrP and infectivity in exposed cultures. We also provide evidence for abnormal PrP deposition in peripheral neuroglial cells from scrapie-infected tgOv mice and sheep. These findings have potential implications in terms of designing new cell systems permissive to prions and of peripheral pathobiology of prion infections.
MH Animals; Cells, Cultured; Female; Ganglia, Spinal/cytology; Mice; Mice, Transgenic; Neuroglia/*metabolism; Neurons/metabolism; Peripheral Nervous System/metabolism; PrPc Proteins/metabolism; PrPsc Proteins/metabolism/*pathogenicity; Research Support, Non-U.S. Gov't; Schwann Cells/metabolism; Scrapie; Sheep
AD
Fabienne Archer, Gregory Perrot, Christelle Langevin, Annick Le Dur, Didier Vilette, Hubert Laude, Unité de Virologie Immunologie Moléculaires; Corinne Bachelin, Anne Baron-van Evercooren, Laboratoire des Affections de la Myéline et des Canaux Ioniques Musculaires, INSERM U546, IFNRS70, CHU Pitié-Salpétrière, Paris; Olivier Andreoletti, Unité Interactions Hôte-Pathogène, Ecole Nationale Vétérinaire, UMR INRA-ENVT, Toulouse, France;
Nathalie Besnard, Jean-Luc Vilotte, Laboratoire de Génétique Biochimique et Cytogénétique, INRA, Jouy-en-Josas
SP englisch
PO USA