NR APUO
AU Fernandez Garcia,A.; Heindl,P.; Voigt,H.; Büttner,M.; Wienhold,D.; Butz,P.; Stärke,J.; Tauscher,B.; Pfaff,E.
TI Reduced proteinase K resistance and infectivity of prions after pressure treatment at 60 degrees C
QU Journal of General Virology 2004 Jan; 85(1): 261-4
PT journal article
AB High hydrostatic pressure is a mild technology compared with high temperatures and is commonly used for food pasteurization. Crude brain homogenates of terminally diseased hamsters infected with scrapie 263K strain were heated at 60 degrees C and/or pressurized up to 1000 MPa for 2 h. Prion proteins were analysed for their proteinase K sensitivity using a Western blot technique. PrPsc pressurized with 500 MPa or above proved to be proteinase K sensitive. To test the remaining infectivity of the pressurized material, hamsters were infected intracerebrally. Results showed a greatly delayed onset of disease (from 80 up to 153 days) when samples had been pressurized at 500 MPa and above. An increase in the survival rate was also observed: 47 % survival over 180 days was seen following infection with homogenates pressurized at 700-1000 MPa.
MH Animals; Brain/metabolism; Disinfection/methods; Endopeptidase K/*metabolism; Hamsters; *Heat; Hydrostatic Pressure; PrPsc Proteins/*metabolism/*pathogenicity; Scrapie/metabolism/mortality; Support, Non-U.S. Gov't
AD veli.fernandez@bfe.uni-karlsruhe.de; Federal Research Centre for Nutrition, Haid-und-Neustr. 9, 76131 Karlsruhe, Germany. Federal Research Centre for Virus Diseases of Animals, Paul-Ehrlich-Str. 28, 72076 Tübingen, Germany.
SP englisch
PO England
SP englisch
PO England