NR AQQL
AU Wurm,S.; Paar,C.; Sonnleitner,A.; Sonnleitner,M.; Höglinger,O.; Romanin,C.; Wechselberger,C.
TI Co-localization of CD3 and prion protein in Jurkat lymphocytes after hypothermal stimulation
QU FEBS Letters 2004 May 21; 566(1-3): 121-5
PT journal article
AB While long-term effects of temperature treatment in respect of, e.g., gene-expression and cellular function have already been studied in some detail, nothing is known on the physiological responses of lymphocytes during short-term hypothermal shifts. In this report, we characterized the effects of such a stimulation using the human lymphocyte cell line Jurkat E6.1 and present evidence that warming from 4 to 37 degrees C for only 2 min is sufficient to cause co-localization of CD3, prion protein and the lipid-raft ganglioside GM1 paralleling lymphocyte activation as observed by Ca(2+) mobilization and mitogen-activated protein kinase-phosphorylation.
MH Actins/metabolism; Antigens, CD3/chemistry/*metabolism; Calcium/chemistry/metabolism; Cell Membrane/metabolism; Cholesterol/deficiency/metabolism; Cyclodextrins/pharmacology; Cytochalasin D/pharmacology; Enzyme Activation; Fluorescent Antibody Technique; G(M1) Ganglioside/chemistry/metabolism; Human; *Hypothermia, Induced; Jurkat Cells; Lymphocyte Activation; Mitogen-Activated Protein Kinases/metabolism; Prions/*metabolism; Protein Structure, Tertiary; Support, Non-U.S. Gov't; T-Lymphocytes/*metabolism
AD Upper Austrian Research GmbH, Center for Biomedical Nanotechnology, Scharitzerstr. 6-8, 4020 Linz, Austria.
SP englisch
PO Niederlande