NR AQTA
AU Hundt,C.; Weiss,S.
TI The prion-like protein Doppel fails to interact with itself, the prion protein and the 37 kDa/67 kDa laminin receptor in the yeast two-hybrid system
QU Biochimica et Biophysica Acta 2004 May 24; 1689(1): 1-5
PT journal article
AB The prion-like protein termed Doppel (Dpl) shows approx. 25% sequence identity with all known prion proteins (PrP). We recently showed that the cellular PrP is dimeric under native conditions, a finding which was confirmed by the investigation of its crystal structure. Human PrP further interacts with its cellular receptor, the 37 kDa/67 kDa laminin receptor (LRP/LR). Here we report that human Doppel fails to interact with (i). itself, (ii). the human 37 kDa/67 kDa LRP/LR, and (iii). the human cellular prion protein (huPrP) in the yeast two-hybrid system. Our findings suggest that Dpl and PrP are not related or are only marginally related with respect to their ligand binding behaviour.
MH Human; Molecular Weight; Prions/chemistry/genetics/*metabolism; Protein Binding; Receptors, Laminin/*chemistry/genetics/*metabolism; Support, Non-U.S. Gov't; Two-Hybrid System Techniques
AD Laboratorium für Molekulare Biologie-Genzentrum-Institut für Biochemie der Ludwig-Maximilians-Universität München, Feodor-Lynen-Str. 25, D-81377 Munich, Germany.
SP englisch
PO Niederlande